P物质对大鼠分离的DRG细胞GABA激活电流的抑制作用  

INHIBITORY EFFECT OF SP ON GABA-ACTIVATED CURRENTS IN FRESHLY ISOLATED RAT DRG NEURONS

作　　者：吴晓平[1] 李之望[1] 樊友珍[1] 

机构地区：[1]同济医科大学实验医学研究中心

出　　处：《生理学报》1994年第6期586-590,共5页Acta Physiologica Sinica

基　　金：国家自然科学基金

摘　　要：本文应用全细胞膜片箝技术，在新鲜分离的大鼠ＤＲＧ细胞上证明：在部分细胞Ｐ物质（１０（－７）－１０（－５）ｍｏｌ／Ｌ）可引起浓度依赖性的内向流（４／２６）；在多数细胞虽未检测到ＳＰ引起的膜电流，但却能对ＧＡＢＡＡ受体激活介导的膜内向流产生抑制效应（１８／２２），并有加速去敏感的作用。本文就有关ＳＰ对ＧＡＢＡ激活电流抑制效应的可能意义进行了讨论。The whole-cell patch-clamp technique was used to record GABA-activated currents in freshly isolated DRG neurons.It was found that application of SP (10-10(-5)mol/L)could induce concentration dependent inward currents in some cells (4/26),while no such current was detectable in the other neurons.However, preapplication of SP could inhibit the inward currents mediated by GABA& receptor(18/22).The inhibitory effect of SP was concentration dependent and reversible,and the desensitization of GABA-activated current could also be enhanced by SP.

关 键 词：P物质 Γ氨基丁酸 背根神经节细胞 

分 类 号：R338[医药卫生—人体生理学]