聚焦色谱法测定肌型肌酸激酶亚型  

Measurement of Creatine Kinase MM Iso-forms by Chroiuatofocusing.

作　　者：孔庆银[1] 杨振华[1] 杨树德[1] 唐志毅[1] 

机构地区：[1]北京医院检验科

出　　处：《生物化学与生物物理进展》1994年第1期83-85,共3页Progress In Biochemistry and Biophysics

摘　　要：报道了测定ＣＫ－ＭＭ亚型的聚焦色谱法，此法简单，快速，结果可靠，线性范围宽，最低检测限（８Ｕ／Ｌ）较正常参考值低，比国外报道的类似方法高６倍以上，分离度亦有改进。测定了２０例健康人血清亚型分布，与文献报道结果相近。该法自动化程度高，已在急性心梗的诊断中实际应用。rapid and sensitive method of isoforms ofCK (EC 2. 7. 3. 2) -MM in human serum bychromatofocusing was reported. The assaysystem involved Mono P (HR5/20) column.fast protein liquid chromatography (FPLC)system and post column reactor. The result ofCK-MM isoforms was obtained within 40 minafter serum application to equilibrated column.The lower limit of sensitivity for CK activity is8 U / L and the linear capacity is 1 7- 2 0 8 5U/L, with CV values of 2. l%. 2. 7% andll. 0% for MM_1. MM_2 and MM_3 respectively.

关 键 词：聚集色谱法 血清诊断 肌酸激酶 

分 类 号：R446.112[医药卫生—诊断学]