Zn对细胞保护作用机理的研究  

Study on the Mechanism of Protective Effet ofZinc to Cells.

作　　者：章净霞[1] 黄萍[1] 徐世文[1] 安丽芝[1] 姚惠英[1] 肖延安 潘巨祥 巢志瑜 朱节清 邹显慷 

机构地区：[1]北京医科大学生物物理教研室,复旦大学核科学系,北京正负电子对撞机同步辐射实验室,上海原子核研究所

出　　处：《生物化学与生物物理进展》1994年第2期147-150,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金

摘　　要：应用扫描质子微探针和同步辐射ｘ荧光分析技术测定了细胞中元素的分布和组成，为确定Ｚｎ是细胞结构成分提供了直接的实验依据。用上述核技术结合有关生化指标，分析测定了正常和损伤细胞（脂质过氧化损伤）中Ｆｅ，Ｚｎ和丙二醛、ＳＨ基含量变化的相互关系。实验结果表明，当细胞发生脂质过氧化损伤时，Ｆｅ含量和丙二醛含量同步增高，而Ｚｎ含量和ＳＨ基量则降低。给细胞补充Ｚｎ后，提高了细胞质膜中的Ｚｎ含量，ＳＨ基量也随之增加，同时丙二醛量降低。提示Ｚｎ保护细胞完整性的作用机理之一是控制脂质过氧化作用。Ｚｎ可保护膜蛋白的ＳＨ基，减少和阻止被Ｆｅ所催化的过氧化反应。On analyzing the distribution and compositionof element in single cell with scanning protonmicroprobe (SPM) and synchrotron radiationX-Ray fluoroscence microprobe (SR-XMF) itshows that zinc is a constituent of cell plasma.The levels of cellular zinc and iron were de-tectecd by above nuclear technique and thecontents of MDA, SH group were estimatedrespectively by biochemical method for bothnormal and injured cell induced an peroxidationdamage by hydroxyl-free radical. It was foundthat MDA content increased, SH group de-creased. as well as Fe/Zn ratio raised duringlipoperoxidation. By supplementation of zincto culture medium, the inhibitory effect of zincon lipoperoxidation was obvious from followingexperiment results that MDA content de-creased, SH group content increased as well asFe/Zn ratio reduced. The results suggest thatzinc plays a role in maintaining the integrity ofthe cell and protects SH group of membraneprotein thus preventing catalytic peroxidationreaction of iron.

关 键 词：锌 细胞 保护 过氧化脂质 

分 类 号：Q27[生物学—细胞生物学]