鲑鱼生长激素基因分泌型表达质粒的构建  

Expression and Secretion of Salmon GrowthHormone From an Escherichia coli SecretionVector.

作　　者：张同海[1] 宋诗铎[1] 赵为诚[1] 陈昆明[1] 祁伟[1] 胡文芝[1] 王培福[1] 方佩华[1] 

机构地区：[1]天津医学院第二附属医院,天津内分泌研究所

出　　处：《生物化学与生物物理进展》1994年第4期338-341,共4页Progress In Biochemistry and Biophysics

基　　金：天津科委自然科学基金

摘　　要：生长激素（ＧＨ）是动物垂体前叶分泌的一种多肽类激素．应用分子重组及ＰＣＲ等技术，构建了一种鲑鱼生长激素基因分泌型表达质粒ｐＯｓＧＨ１５３，使编码鲑鱼生长激素成熟肽的序列克隆在大肠杆菌分泌型表达载体ＰＩＮ－Ⅲ－ｏｍｐＡ内，直接位于编码大肠杆菌外膜蛋白Ａ信号肽序列的下游，在Ｌｐｐ－Ｌａｃ杂合启动子控制下，经ＩＰＴＧ诱导，分子量约２３０００的鲑鱼生长激素在大肠杆菌中获得高效表达，该产物具有天然鲑鱼生长激素的免疫活性，直接分泌到细胞周质，而信号肽被自动剪除．cDNA fragment encoding mature salmongrowth hormone (sGH) was successfully am-plified by polymerase chain reaction and the re-striction recognition sequences were respec-tively introduced into the 5' end and 3' end ofthe amplified fragment. The recombinant se-cretion vector pOsGH153 containing genescoding for the E. coli ompA signal peptide andmature sGH was constructed and confirmed byrestriction enzyme analysis and Southern blothybridization with the oligonucleotide probe.Upon induction of IPTG, the sGH was ex-pressed in E. coli cells and was secreted intothe E. coli periplasma.

关 键 词：鲑鱼 生长激素 分泌型 表达质粒 

分 类 号：Q959.466[生物学—动物学]