小鼠全血中谷胱甘肽过氧化物酶活力的微量测定法  

Direct Micro Determination of GlutatbionePeroxidase Activity in Mice Blood.

作　　者：荣征星[1] 刘慧中[1] 鲍景奇 陈红专[1] 孙玉燕[1] 孙琛[1] 

机构地区：[1]上海第二医科大学药理学教研室,上海市长宁区中心医院

出　　处：《生物化学与生物物理进展》1994年第4期362-366,共5页Progress In Biochemistry and Biophysics

摘　　要：报道了用分光光度计法直接测定小鼠全血中谷胱甘肽过氧化物酶ＧＳＨ－ＰＸ活力的方法．取血样１０μｌ，４２３ｎｍ为测定波长，三氯醋酸为蛋白沉淀剂，在ｐＨ６．５，３ｍｉｎ的酶反应条件下，反应剩余的谷胱甘肽和其与ＤＴＮＢ试剂反应生成的颜色产物成线性相关．该法灵敏度高，重复性好，所需仪器简单。direct micro determination of glutathioneperoxidase (GSH-PX) activity with spec-trophotometry was developed. Conditions ofthe assay were studied in detail. 10μl bloodsample was diluted with distilled water andtreated with 10% TCA to remove protein.There was a good linearity between DTNBproduct and concentration of GSH after 3 min-utes enzymatic reaction at the temperature of37℃ in PH 6.5 solution. The method hadhigher sensitivity, better reproducibility. Itmay became useful tool for analyzing GSH-PXin scientific research and clinical work.

关 键 词：谷胱甘肽 过氧化物酶 分光光度法 

分 类 号：R446.112[医药卫生—诊断学]