关于吖啶橙测定溶酶体H^＋—ATPase转运H^＋的研究  

Study of the Measurements with AcridineOrange in Protons Transportation of LysosomeH￣+-ATPase.

作　　者：张过江[1] 王嘉[1] 姚军兰 刘晓麒[1] 

机构地区：[1]中国科学院生物物理研究所

出　　处：《生物化学与生物物理进展》1994年第6期539-543,共5页Progress In Biochemistry and Biophysics

基　　金：所长择优课题基金

摘　　要：揭示了吖啶橙的吸收光谱和荧光光谱对其浓度依赖性上的区域性特征，分析了测定溶酶体Ｈ￣＋转运时合理选用吖啶橙浓度及溶酶体用量的重要性、机理和原则，探讨了其与溶酶体的温育时间和Ｋ￣＋／Ｈ￣＋交换对测定Ｈ￣＋转运的明显影响。he different range characteristics of acridineorange in absorption and fluorescence is depen-dent on its concentrations.The importanceand mechanism as well as principles of choo-sing correct acridine orange concentration andrational amounts of lysosome in the assay ofprotons pumping are discussed. The obviousinfluences of the incubation time of acridineorange with lysosome and the K￣+/H￣+ ex-change upon the measurements of proton trans-port are studied.

关 键 词：吖啶橙 溶酶体 质子 腺苷三磷酸酶 氢离子 

分 类 号：Q－33[生物学]