盐酸胍对大麦类囊体膜能量分配和电子传递的影响  

Effect of Guanidine Hydrochloride on Energy Distribution and Electron Transport of Barley Thylakoid Membranes

作　　者：于晖[1] 张正东[1] 马桂芝[1] 

机构地区：[1]中国科学院研究生院生物教学部,中国科学院植物研究所

出　　处：《生物化学与生物物理学报》1994年第1期73-79,共7页

基　　金：中国科学技术大学资助

摘　　要：本文以大麦（ＨｏｒｄｅｕｍｖｕｌｇａｒｅＬ．）叶片为实验材料，研究了盐酸胍修饰对类囊体膜能量分配及电子传递的影响。结果表明：盐酸胍处理类囊体膜，室温下Ｆ６８５荧光强度，随着盐酸胍浓度的增加而逐渐下降．盐酸胍处理导致类囊体膜在低温（７７Ｋ）下Ｆ６８６／Ｆ７３６比值下降，并随着盐酸胍浓度的增加而加剧。盐酸胍处理抑制类囊体膜以Ｈ２Ｏ为电子供体的ＤＣＩＰ光还原速率和Ｃｈｌａ诱导荧光产率，这种抑制作用可分别为加入ＰＳＩＩ的人工电子供体ＤＰＣ和ＮＨ２ＯＨ部分恢复。盐酸胍处理刺激类囊体膜以Ａｓｃ／ＤＣＩＰ为电子供体的甲基紫精光还原速率。以上结果表明，用盐酸胍修饰类囊体膜有利于激发能从ＰＳＩＩ向ＰＳＩ分配。Effects of guanidine hydrochloride (GuHCl) on the energy distribution and the electron tranoprt of barley thylakoid membranes have been investigated. The following were discovered: GuHCl decreases F685 emission at the room temperature,and with the increase of the concentration of GuHCl the emission intensity declined. GuHCl induces a significant decrease of F685/F736 ratio at 77 K, and with the increase of the concentration of GuHCl this decrease is enhanced. GuHCl inhibits the rate of photoreduction of DCIP with H2O as the electron donor and the yield of the variable Chla fluorescence. The inhibitions respectively could be restored by the addition of artificial electron donor of PSII, DPC and NH2OH.GuHCl stimulates the rate of methyl viologen photoreduction (with DCIP/Asc. as the electron donor system).It is suggested that the protein on the thylakoid membranes was modified by GuHCl, which caused the excitation energy redistribution from PSII to PSI.

关 键 词：大麦 盐酸胍 类囊体膜 电子传递 

分 类 号：Q947[生物学—植物学]