超氧自由基与嘧啶碱基及其核苷反应的ESR研究  

ESR Studies on Reaction of Superoxide Radical (O_2￣-)with Pyrimidine Bases or Pyrimidine Nucleosides

作　　者：王金富[1] 李建华[1] 戴柏青[1] 阎作琨 恽勤[1] 

机构地区：[1]哈尔滨师范大学化学系,中国科学院长春应用化学研究所

出　　处：《生物化学与生物物理学报》1994年第4期377-382,共6页

摘　　要：用自旋捕集技术和ＥＳＲ方法，以ＭＮＰ为捕集剂，研究了紫外线辐照核黄素产生超氧自由基（Ｏ）等活性氧与嘧啶碱基及其核苷的反应。确定了尿嘧啶、胞嘧啶和胸腺嘧啶及其核苷所产生的自旋加合物自由基的类别，讨论了自由基的形成机制，揭示了超氧自由基与嘧啶碱基及其核苷的反应不是直接进行的，而是通过羟基自由基（ＯＨ）来实现的。The reaction of superoxide radioal(O)with pyrimidine bases or prymidine nucleosides in aqueous solution of riboflavine containing MNP have been studied with the spin trapping and ESR technique.The radicals of O spin adduct yielded by pyrimidine bases of uracil, cytosine,thymine and their nucleosides are certified. The formation mechanism of these radicals is also disscused. It is indicated that superoxide radioal can not react directly with pyrimidine bases or pyrimidine nucleosides but reacts through the hydroxyl radicals(OH).

关 键 词：超氧 自由基 嘧啶 ESR 自旋捕集 

分 类 号：Q505[生物学—生物化学]