旋扭山绿豆（Desmodiumintortum）凝集素的纯化与特性  

Purification and Characterization of the Lectin from Desmodium intortum

作　　者：彭建宗[1] 程双奇[1] 陈兆平[1] 莫熙穆 

机构地区：[1]华南师范大学生物系

出　　处：《生物化学与生物物理学报》1994年第6期671-674,共4页

摘　　要：旋扭山绿豆（Ｄｅｓｍｏｄｉｕｍｉｎｔｏｒｔｕｍ）凝集素的纯化与特性彭建宗，程双奇，陈兆平，莫熙穆（华南师范大学生物系，广州５１０６３１）关键词凝集素；旋扭山绿豆；亲和层析；糖蛋白豆科植物和根瘤菌之间的共生结瘤固氮关系早有报道。例如大豆只被Ｂ．仰。６。...The lectin in Desmodium intortum (DIL)was purified by affinity chromatography and gel filtration. The purified DIL exhibited a single band of molecular weight about 32 kd by SDS-PAGE. It is a glycoprotein and has a sugar content of 6.2%.DIL is an excellent mitogen. The transformation ratio of human peripheral blood lymphocytes is 87% and the cell division ratio is 15%.The results with 13 strains examined by the test of fluorescent label showed that FITO-DIL could combine specifically with 9 strains that nodulated Desmodium intortum but could not combine any of the other 4 strains that didn't nodulate D. intortum.It revealed a positive biological correlation between the lectin-binding capacily and the nodulating ability,illustrating that the DIL is important in the host-specific infection process which leads to nitrogen fixing symbiosis.

关 键 词：凝集素 旋扭山绿豆 亲和层析 糖蛋白 

分 类 号：Q949.751.9[生物学—植物学]