镱和铕诱导金属硫蛋白生成的初步研究  

Study on the Induction of Ytterbium and Europium to the Synthesis of Liver Metallothionein of Rabbit

作　　者：肖广庆[1] 唐任寰[1] 王素云[1] 曹同庚 李令媛[1] 

机构地区：[1]北京大学技术物理系,北京大学生物系

出　　处：《生物化学杂志》1994年第1期93-97,共5页

基　　金：国家自然科学基金;中科院水生生物所开放实验室资助

摘　　要：本文研究了不同剂量的稀土元素镱和铕，通过皮下注射对兔肝中金属硫蛋白生成的影响。用ＳｅｐｈａｄｅｘＧ５０柱对兔肝提取液进行分离，收集的组分用紫外分光光度法、原子吸收光谱法和ＥＬＩＳＡ法进行分析。实验结果表明，低剂量（１－３ｐｐｍ）的镱和铕不能明显地诱导兔肝内金属硫蛋白的合成，而高剂量（１０－４０ｐｐｍ）的镱和铕可以诱导兔肝金属硫蛋白合成。Rabbits were exposed to Rare Earth Elements(Ytterbium and Europium)through hypodermic injection, the liver metallothionein (MT) was separated on Sephadex G-50 columns,and the fractions were collected and analyzed with ELISA, ultraviolet and atomic absorption of zinc method. The results indicated that low dose(1-3 ppm) Yb and Eu could not induce the synthesis of MT, but high dose(10-40 ppm)stimulated the synthesis of MT in liver of rabbit.It was also found that there were no difference in zinc concentration of high molecular and low molecular weight fractions among control, low dose and high dose groups, but the zinc concentration in MT fraction of high dose groups were greatly increased, Yb and Eu have induced the synthesis of MT in liver of rabbit.

关 键 词：镱 铕 金属硫蛋白 稀土族 

分 类 号：Q510.3[生物学—生物化学]