人血红细胞蛋白质酪氨酸磷酸酶（PTPP）的研究Ⅰ·PTPP在红细胞胞浆和膜中的分布及其影响因素  

Research for Protein Tyrosine Phosphatases (PTPPs) of Human Erythrocytes Ⅰ· Distribution and Its Influential Factors of PTPPs in Cytosol and Membrane

作　　者：朱茂祥[1] 吴国利[1] 

机构地区：[1]北京师范大学分子生物学及生物化学研究室,军事医学科学院放射医学研究所

出　　处：《生物化学杂志》1994年第2期169-173,共5页

基　　金：国家自然科学基金

摘　　要：通过测定红细胞胞浆及膜中的ＰＴＰＰ活性，发现人正常血红细胞中胞浆ＰＴＰＰ活性约占红细胞ＰＴＰＰ总活性的７０－８０％，膜中只有约２０－３０％的ＰＴＰＰ活性。很多因素诸如：病变、ＰＨ值、温度、离子强度、细胞贮存时间以及药物等，对ＰＴＰＰ在胞浆与膜中的分布有影响。可以推测：膜上的ＰＴＰＰ能通过某种机制解离下来，进入胞浆；相反的过程，胞浆中的ＰＴＰＰ也能通过某种机制与膜结合。这种偶联与去偶联的具体机制及其生理功能还有待进一步探索。Through assaying the protein tyrosine phosphatases(PTPPs) activities of cytosol and membrane of human erythrocytes, we found that there were 70-80% PTPP activities of normal red blood cell in the cytosol, and only 20-30% PTPP activities in the membrane.Many factors such as: temperature, pH, store time, some reagents and pathological changes etc. can influence the distribution of PTPP activities between cytosol and membrane. These suggest that PTPP of membrane may be released and enter into cytosol through a certain mechanism; On the other hand, the PTPP of cytosol may also be associated with membrane.

关 键 词：红细胞 胞浆 蛋白质 酪氨酸磷酸酶 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学]