人血红细胞蛋白质酪氨酸磷酸酶（PTPP）的研究──Ⅲ．胞浆PTPP与红细胞膜的结合及其结合后的主要性质  

Research for Phosphotyrosyl Protein Phosphatase (PTPP) of Human Erythrocytes Ⅲ. Association of Cytosolic PTPP Asssociated with Membrane and Properties of PTPP Associated with Membrane

作　　者：朱茂祥[1] 吴国利[1] 

机构地区：[1]北京师范大学分子生物学及生物化学研究室

出　　处：《生物化学杂志》1994年第3期279-284,共6页

基　　金：国家自然科学基金

摘　　要：用部分纯化的胞浆ＰＴＰＰ分别与去ＰＴＰＰ活性的红细胞血影（ｄＰＴＰＰ－Ｇｈｏｓｔ）和内向外翻红细胞膜（ｄＰＴＰＰ－ＩＯＶ）结合，结果发现：胞浆ＰＴＰＰ不仅能与红细胞膜结合，而且与ｄＰＴＰＰ－ＩＯＶ的结合能力强于与ｄＰＴＰＰ－Ｇｈｏｓｔ的结合。很多因素，诸如：温度，ｐＨ，离子强度，结合时间及某些试剂等不仅对ＰＴＰＰ与膜的结合有影响，且对ＰＴＰＰ与ｄＰＴＰＰ－１０Ｖ结合的影响比与ｄＰＴＰＰ－Ｇｈｏｓｔ结合的影响更为显著。当胞浆ＰＴＰＰ与红细胞膜结合后，其性质发生变化，这主要表现在稳定性增强；Ｋｍ减小；Ｚｎ￣（２＋）、ＶＯ等的抑制作用及ＥＤＴＡ、甘油等的激活作用都明显减弱，甚至向相反的作用转化。此外，红细胞膜的磷酸化／脱磷酸化对ＰＴＰＰ的结合有影响，这暗示磷醚化作用对此可能具有调节意义。Using partially purified cytosolic PTPP to combine with dPTPP-ghost and dPTPP-IOV membrane of human erythrocytes, respectively, we obtained these results: not only the cytosolic PTPP could associate with membrane, but the ability with dPTPP-IOV membrane was greater than that of PTPP doing with dPTPP-ghost. Many factors such as temperature, pH, ionic strength,association time and some reagents etc. could influence the association,and the effects on the association of PTPP with dPTPP-IOV membrane were more apparent than that of it with dPTPP-ghost. The properties of PTPP associated with membrane (dPTPP-IOV) were different from cytosolic dissociating PTPP. For example, the heat-stability was increased; the Km was decreased; and the inhibition of Zn￣(2+),VO and activtion of EDTA, glycerol etc. were apparently weakened. Besides, by treating the dPTPP-IOV membrane with PTPP, PTK, CIP etc. respectively, we found that its association abilitys with cytosolic PTPP was weakened. This indicates that phosphorylation / dephosphorylation may control the association of PTPP with membrane.

关 键 词：PTPP 红细胞 膜 胞浆 血液 

分 类 号：Q592.1[生物学—生物化学]