两种新的激活γ珠蛋白基因的中药──盐酸山莨菪碱（654－2）和三尖杉酯碱  

Two Drugs Stimulate γ-Globin Gene Expression in K562 Cells

作　　者：杨克恭[1] 何平[1] 陈松森[1] 贾佩臣[1] 梁植权[1] 

机构地区：[1]中国医学科学院基础医学研究所

出　　处：《生物化学杂志》1994年第3期285-290,共6页

基　　金：国家自然科学基金;高校博士学科点专项科研基金

摘　　要：检测了３７种中药对Ｋ５６２细胞生长及血红蛋白合成的影响，发现盐酸山莨菪碱（６５４－２）和三尖杉酯碱可以使Ｋ５６２细胞的血红蛋白细胞增加３倍，细胞平均血红蛋白含量增加４倍，γ珠蛋白ｍＲＮＡ的表达分别增加５０％和４０％。电泳结果显示ＨｂＦ和γ珠蛋白肽链。Two drugs,Anisodamine hydrochloride (AAHC) and Harringtonine (HRT), are able to induce an increase of the expression of γ-globin gene in K562 cells. After 2 days of induction by AAHC (10-3mg/mL) and HRT (10-4mg/mL) , benzidine positive cells were increased by 3 fold, with the hemoglobin contents of 2. 16pg and 2. 10pg per cell respectively,in contrast to 0. 41pg per cell of the control. The amount of γ mRNAs were also increased by 50% and 40% above the level found in the control respctively after AAHC and HRT induction. In addition,we have analysed the DNA methylation in K562 cells treated with AAHC and HRT,and the result suggests that the mechanism leading to an incrase of γ gene expression may be associated with an erythroid differentiation.

关 键 词：盐酸 山莨菪碱 三尖杉酯碱 珠蛋白 

分 类 号：R285[医药卫生—中药学]