化学诱变抗体模拟谷胱甘肽过氧化物酶  

Artificial Imitation of Glutathione Peroxidase by Using ChemicalMutation Method

作　　者：丁兰[1] 朱振齐[1] 罗贵民[1] 孙启安 杨同书[1] 沈家骢[1] 

机构地区：[1]吉林大学酶工程实验室

出　　处：《生物化学杂志》1994年第3期296-299,共4页

摘　　要：用诱变剂苯甲基磺酰氟（ＰＭＳＦ）活化兔抗人ＩｇＭ（Ｆｃμ）片段上特殊活性部位的丝氨酸（Ｓｅｒ）残基，经硒化氢（Ｈ_２Ｓｅ）处理，则将丝氨酸转变成硒代半胱氨酸（ＳｅＣｙｓ）。诱变后的抗体具有谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性，其活性为世界最好的ＧＳＨ－Ｐｘ模拟物ＰＺ５１的７０多倍。抗体效价为１：８，与没诱变的抗体相似。Alcohlic groups of serine(Ser) that are located in anti-human IgM(Fcμ) (rabbit Ig fraction) specific site are selectively activated by phenylmethylsulfonyl fluoride (PMSF) and displaced with hydrogen selenide (H2Se). Thus, Ser residues can be chemically converted into selenocysteine (SeCysh). The modified antibody has glutathione peroxidase (GSH-Px) activity which is seventy times more than that of PZ51, the best mimic known in the world.The titer of modified antibody is similar to that of the unmodified antibody.

关 键 词：谷胱甘肽 过氧化物酶 化学诱变 

分 类 号：Q550.4[生物学—生物化学]