UV辐射所致HL－60细胞DNA不均一修复的研究  

Study on Heterogeneous DNA Repair in UV-Irradiated HL-60 Cells

作　　者：严涛[1] 崔立斌[1] 孙丽亚[1] 魏康[1] 方允中[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《生物化学杂志》1994年第3期357-362,共6页

基　　金：国家自然科学基金

摘　　要：在特定基因水平检测了ＵＶ照射后ＨＬ－６０细胞的ＤＮＡ修复。结果显示活性转录的ｃ－ｍｙｃ基因的修复水平明显高于非活性转录的β珠蛋白基因和全基因组。而进一步应用链专一性ＲＮＡ探针检测，发现ｃ－ｍｙｃ基因中的转录链和非转录链的修复效率没有明显差异。上述结果表明，ＨＬ－６０细胞能够对活跃表达基因的损伤进行选择性高效修复，但不能对活跃表达基因中的转录链进行进一步的选择性修复。To investigate DNA repair further, we detected the gene specific repair of HL-60 cell after UV irradiation, and find that the repair of actively transcribed c-myc gene fragment is much more efficient than nonactive β-globin gene and genome overall.About 79% of the dimer are removed from c-myc gene fragment within 24h after irradiation, but only 46% from β-globin gene and 56% from genome overall. Furthermore, our test results show that there is no significant difference between the repair efficiency of the transcribed and nontranscribed strands of c-myc gene. This suggests that the UV sensitivity of HL-60 cell comes from the defect in transcribed-strand-selectively repair.

关 键 词：HL60细胞 DNA 修复 紫外线 

分 类 号：Q506[生物学—生物化学]