降血脂药物（DEHP）对酰基CoA氧化酶基因反式作用因子的影响  

The Effect of Hypolipidemic Drug(DEHP) on the Trans-acting Factor(s) for Gene of Acyl-CoA Oxidase

作　　者：温进坤[1] 胡静[1] 魏素珍[1] 贾根深[1] 

机构地区：[1]河北医学院基础医学研究所生化室

出　　处：《生物化学杂志》1994年第6期733-736,共4页

摘　　要：从对照和用ＤＥＨＰ处理的大鼠肝脏提取核蛋白，以含酰基ＣｏＡ氧化酶（ＡＯＸ）基因表达调控部位的ＤＮＡ片段和该基因的不同蛋白结合位点的ＤＮＡ片段作为核蛋白结合反应的探针，通过凝胶电泳迁移率改变实验和Ｓｏｕｔｈｗｅｓｔｅｒｎ印迹分析检查了ＤＥＨＰ对ＡＯＸ基因反式作用因子的影响。结果表明，降血脂药物ＤＥＨＰ可显著增加ＡＯＸ基因反式作用因子的含量和（或）与基因的结合活性，在转录水平上促进基因的表达。The liver nuclear proteins were extracted from the control and DEHP-treated wistar rats. Utilizing the radioactive DNA fragments containing upstream regulatory elements of AOX gene and protein-binding site A and B DNA fragments as probes, we have investigated the effect of hypolipidemic drug(DEHP) on the trans-acting factor(s) for AOX gene by electrophoretic band-shift experiment and Southwestern blot analysis. The results suggest that rat liver nuclear extracts contain a protein of 100kD which exhibits specific binding activity for the binding site B ,and that the 100kD protein was markedly increased in the liver of DEHP-treated rats. These data provide evidence for the presence of the trans-acting factor(s)that can be induced by DEHP in rat liver nuclei, which may be involved in the molecular events responsible for transcriptional regulation of AOX gene.

关 键 词：反式作用因子 降血脂药 酰基 辅酶A氧化酶 基因 

分 类 号：R972.6[医药卫生—药品]