Ri质粒转化桔梗再生植株的研究  

Plant Regenration from Platycoden Grandiflorum Tarnsgormed by Agrobacterium Rhizogenes

作　　者：刘伟华[1] 姜静[1] 谢桂芹[1] 徐香玲[1] 李集临[1] 王毅[1] 

机构地区：[1]哈尔滨师范大学生物系,吉林农业大学特产园艺系

出　　处：《生物技术》1994年第2期24-29,共6页Biotechnology

基　　金：省自然科学基金

摘　　要：本文用具Ｒｉ质粒的发根农杆菌（Ａｇｒｏｂａｃｔｅｒｕｍｒｈｉｚｏｇｅｎｅｓ）的不同菌株通过叶盘法和直接注射法对药用植物桔梗（Ｐ．ｇｒａｎｄｉｆｌｏｒｕｍＡＤＣ）进行感染试验，系统地研究了发根农杆菌诱导桔梗产生毛状根的能力及影响因素，阐明了桔梗的Ｒｉ质粒Ｔ－ＤＮＡ转化体多样性的原因，为转化体的筛选与鉴定提供了依据。还探讨了Ｒｉ质粒做为植物基因工程载体系统的可行性，为进一步开展植物基因工程载体系统的构建提供基础资料。Ri -plasmid of Agrobacterium rhizogenes was directly injected into medicinal plant P. grandiflorum or their leaf dishes. Capability and condition of producing hairy root of P. grandiflorum were systema tically studied. The diverse causes of T-DNA of Ri-plasmid of P. grandiflorum transformation were more expounded systematically so that the foundaction is provided for selectting and identiffing the transformation. The possibility of using the Ri-plasmid as a vector for plant gene engineering was inverstigated. These base data are useful in studing conStruction of vector system for plant gene engineering.

关 键 词：发根农杆菌 转化体 植株再生 桔梗 

分 类 号：S188[农业科学—农业基础科学]