灵芝多糖对人脐血LAK细胞活性的影响  

Effect of Polysaccharide of G. Lucidum on Lak Cell Activity of Human Cord Blood

作　　者：徐新[1] 曹容华[1] 侯桂华[1] 

机构地区：[1]山东医科大学微生物免疫室,山东泰安市泰山医学院微生物教研室

出　　处：《生物技术》1994年第2期33-35,23,共4页Biotechnology

摘　　要：本文研究了灵芝多糖（ＧＬＰ）对人脐血ＬＡＫ（ＣＢ—ＬＡＫ）细胞活性的影响，结果发现，单独ＧＬＰ能刺激人脐血单个核细胞（ＣＢＭＣ）增殖，但不能诱导ＬＡＫ活性，当与５０ｕ／ｍｌｒＩＬ—２伍用时，可增殖ＣＢ—ＬＡＫ细胞诱导活性，不同剂量ＧＬＰ（０．５—１００μｇ／ｍｌ）影响作用不同，以１０μｇ／ｍｌ浓度最好．在不同浓度ｒＩＬ—２（１０—１００ｕ／ｍｌ）诱导ＣＢ—ＬＡＫ细胞过程中加入ＧＬＰ（１０μｇ／ｍｌ），可明显提高细胞增殖能力，减少ｒＩＬ—２用量。ＧＬＰ亦能促进效应阶段ＣＢ—ＬＡＫ细胞对Ｒａｊｉ肿瘤靶细胞的杀伤作用（Ｐ＜０．００１）。由此看出，ＧＬＰ具有增强ＣＢ—ＬＡＫ细胞活性的作用，是一很好的生物反应调节剂（ＢＲＭ），有必要进行深入的研究。Effect of polysaccharide of G. lucidum(GLP) on activity of LAK cells derived from human cord blood(CB-LAK) was studied. The results indicated that: GLP alone could enhance the proliferation of CBMC without induction on cytotoxicity. CB-LAK activity could be augmented by GLP in different doses(0. 5-100μg/ml) in the presence Of rIL- 2 (50u/ml). The best dosage of GLP was 10 g/ml. GLP (10μg/ml) could raise the proliferative ability of CB-LAK cells induced with different concentration of rIL- 2 (10T100u/ml) and also promote CB-LAK cytolytic activity against 51Cr - labeled Rail target cells during effector phase. This results suggest that GLP is a good biological response modifiers(BRM). It is nessary to give further study.

关 键 词：灵芝 多糖 人类 脐血 杀伤细胞 

分 类 号：R979.5[医药卫生—药品]