脂质体过氧化对DNA的损伤研究  

STUDY OF DNA DAMACE INDUCED BY LIPOSOME PEROXIDATION

作　　者：曹恩华[1] 刘晓麒[1] 朱曰荣[1] 

机构地区：[1]中国科学院生物物理研究所

出　　处：《生物物理学报》1994年第1期68-74,共7页Acta Biophysica Sinica

基　　金：国家自然科学基金

摘　　要：研究了以脂质体为材料的脂质过氧化引起的ＥＮＡ损伤，同时检测了脂质过氧化程度与ＤＮＡ受损情况。结果表明：在脂质体过氧化程度中，ＤＮＡ的增色效应，对核酸酶的敏感程度，ＤＮＡ双链百分含量和ＤＮＡ－溴乙锭复合物的荧光强度随着氧化时间的增加而降低。在四种碱基中，鸟嘌呤损伤最严重。多种自由基清除列实验表明：脂质过氧化所产生的羟基自由基和单线态氧可能是引起ＤＮＡ氧化损伤的重要因素。DNA damage induced by lipopsome perexidation studied by perexidation and DNAdamage de sncously. The adults shoal that during liposome peroxhation, thehyperehrofority of DNA following thenn Odenaturation, sensibility of DNA to DNase I digeStion, conthe of double-sound DNA and fluo infinity of DNA-EBcomplex dec with moor of reaction theing the four usual bases, guanine wasdamage most seriously than the others. Studies of DNA damage induced by liposomeperoxidation in the pothri of various active oxygen quencheIS shoal that hydroxyl radicalsand singlet oxygen produced in the pro of liposome peroxidation were probably boortantfactors tO DNA damage.

关 键 词：脂质体 DNA 损伤 过氧化脂质 生物膜 

分 类 号：Q734[生物学—分子生物学]