人精浆甘油－3－磷酸胆碱和总唾液酸量变化的研究  

Study on the Sialic Acid and Glycerol-3-phosphorylcholine Contents in Seminal Plasma of the Fertile and Infertile Chinese

作　　者：丁之德[1] 吴宏伟[1] 鲁梅格[1] 吴明章[1] 

机构地区：[1]上海第二医科大学组织胚胎学教研室

出　　处：《生殖与避孕》1994年第4期302-305,共4页Reproduction and Contraception

摘　　要：我们用酶法和过碘酸－硫代巴比妥酸法对５０例正常生育力男性和１６３例四种不育病人精浆甘油－３－磷酸胆碱（ＧＰＣ）、总唾液酸（ＴＳＡ）量进行测定后发现：１．正常男性与无精症（包括分泌性和阻塞性）、少精症精子活动率低下等精浆ＧＰＣ量有显著性差异（Ｐ＜０．０１），含量分别为２．３２±０．６６、１．７２±０．４２、０．８９±０．２６、１．３２±０．４６和１．７５±０．５２ｍｍｏｌ／Ｌ；２．正常男性与精子活动率低下、阻塞性无精等精浆ＴＳＡ量有显著性差异（Ｐ＜０．０１），而与少精和分泌性无精等差异不显著（Ｐ＞０．０５），含量分别为３８８．１６±９８．４５、４６２．６３±８０．６８、２９４．５５±９５．３７、３４９．０７±１０６．２２和３５８．５１±１１９．６２ｍｇ／Ｌ。ＧＰＣ和ＴＳＡ与精子在附睾内成熟即精子受精能力的获得与维持关系密切，精浆ＧＰＣ和ＴＳＡ的变化可能与部分男性不育有关。The concentration of glycerol-3-phosphorylcholine (GPC) and total static acid(TSA) levels are estimated in seminal plasma from 50 fertile and 163 infertile Chinese by the enzymatic and thiobarbituric acid methods. The restllts show that the contents of GPC and TSA are 2. 32±0. 66mmol/L, 388. 14±98. 45 mg/L (X±SD) in the normozoospprmic specimens (50 cases ). In the asthenospermic specimens and the azoospermic Ⅱ (ductal obstruction), the contents of GPC and TSA are significantly different (1. 75±0. 52 mmol/L, 462. 63±80. 68 mg/L, 50 cases 1 0. 89±0.26 mmol/L, 294. 55±95. 37 mg/L. 35cases, P<0. 01).There are some significant differences between the normozoospermic specimens and the oligospermic or the azoospermic I (secretory failure) specimens in GPC content (1. 32±0. 46 mmol/L, 50 cases; 1. 72±0. 42 mmol/L, 28 cases, P<0. 01 ),' but there are no significant differences in TSA content (349. 07±106. 22 mg/L,358. 51±119. 62 mg/L. P>0. 05).In the male reprodtICtive system, the GPC and static acid are synthesized and secreted by the epididymal epithelium. Their levels in seminal plasma may influcence maturation of spermatozoa in the epididymis and be involved in the secretion of epidldymal epithelium. They may be some important indicators of the epididymal function and it suggests that different levels of GPC and TSA in seminal plasms be associated with the male infertility.

关 键 词：唾液酸 精浆 不育症 男性 

分 类 号：R698.202[医药卫生—泌尿科学]