小鼠胸腺树突状细胞系自发分泌多种类型细胞因子  

CYTOKINE PRODUCTION BY MTSC 4 CELLS, AN ESTABLISHED MOUSE THYMIC DENDRITIC CELL LINE

作　　者：刘祝公[1] 陈慰峰[1] 曹良弦[1] 庞学文[1] 张平夏[1] 余青[1] 

机构地区：[1]北京医科大学免疫学教研室,北京100083

出　　处：《实验生物学报》1994年第1期91-101,共11页Acta Biologiae Experimentalis Sinica

基　　金：中华医学基金会洛氏基金;国家自然科学基金

摘　　要：建成小鼠胸腺基质细胞系,命名为MTSC4,经鉴定分析认为是DC来源。在无外来刺激条件下,MTSC 4细胞可自发分泌多种类型细胞因子,已检测到的有IL-1,IL-6,IL-7,CSF,IFN及趋化因子(CF)等。其中IL-6,IFN为较高水平分泌量,IL-1,CF为中等水平,IL-7、CSF为较低水平分泌量。MTSC4不能自发分泌IL-3及TNF_α。MTSC4的建立有利于分析胸腺选择特别是阴性选择的机理,及细胞因子在其中的作用,也可用于研究自分泌因子的网络调节。The cytokine production by MTSC 4 cells, a murine thymic dendritic cell line established in our laboratory, has been investigated. Without exogenous stimulation, the MTSC 4 cells constitively produce multiple types of cytokines, including IL-1, IL-6, IL-7, CSF, IFN and Chemotactic factor (CF). Of which, IL-6 and IFN were abundant; IL-1 and Chemokine, moderate: IL-7 and CSF at low level. MTSC4 cells could not produce detectable either IL-3 of TNFα. The establishment of MTSC 4 may be useful in the analysis of the mechanism of thymus selection, and in the study of the network regulation of au-tocrined cytokines.

关 键 词：胸腺 树突状细胞 细胞因子 分泌 

分 类 号：R392.12[医药卫生—免疫学]