黑热病患者尿中循环抗原的检测及识别抗原分子的研究  

DETECTION AND RECOGNIZATION OF CIRCULATING ANTIGEN IN URINE FROM KALA-AZAR PATIENTS

作　　者：胡孝素[1] 雷莉[1] 林芳清[1] 阚兵[1] 陈建平[1] 何生全 

机构地区：[1]华西医科大学寄生虫学研究室,四川省南坪县人民医院内儿科

出　　处：《实用寄生虫病杂志》1994年第1期1-4,共4页Journal of Practical Parasitic Diseases

基　　金：国家自然科学基金;纽约中华医学基金

摘　　要：“致死性疾病”在苏丹蔓延。我国黑热病亦回升。急迫需要建立新的、对病人无损伤的诊断方法。作者等已建立用血清ＭｃＡｂ－ＡＳＴ诊断黑热病的方法。本文报道用同法检测病人尿中循环抗原的结果。对１７例确诊黑热病患者尿液的检测，１６例呈阳性反应，阳性率９４％。正常人对照３０例均呈阴性结果。１５／１５例黑热病息者血清ＭｃＡｂ－ＡＳＴ显阳性。同一病倒尿样、血清反应的阳性程度相近。取尿液较之任何采样途经都更为简便，特别是对婴幼儿发病率高的山丘疫区，更为适合。免疫印迹法显示识别出二条特异区带。其分子量分别为４５ｋＤ和２２ｋＤ。进一步鉴定出黑热病患者尿中的Ｌ．ｄ抗原。系国内外首次报道。In recent years, the frequency of VL is increasing in several parts of China. It is an urgent need for development of new, noninvasive methods for diagnosis of kala-azar. The paper firstly reports experiments to detect L. donovani (L. d.) circulating antigen in urine of kalaazar patients by McAb-AST. Of tEe 17 urine samples tested, 16 showed positive findings with a positive rate of 94%, while all of normal controls (30 urine samples) gave negative results.15/15 sera samples of patients showed positive by McAb-AST. The color reaction shown by the detectable antigen in urine and serum was similar. It is suggested by our results that the urine sample may be considered as a good candidate in diagnosis of kala-azar by its simple and more convenient than the other sources of specimens, especially in the hill endemic areas with high morbidity among children.Western blots (immunoblots) analysis of L. d. circulating antigen in urine revealed 2 specific bands with molecular weights of 45 kD and 22 kD. No band was shown by normal urine.The existence of circulating antigen in urine of kala-azar patients was further confirmed.

关 键 词：黑热病 尿 循环抗原 免疫印迹 

分 类 号：R531.6[医药卫生—内科学]