鱼类HGPRT缺陷型细胞的诱变和筛选  

THE INDUCTON AND SELECTION OF HGPRT-DEFICIENT CELLMUTANTS IN FISH

作　　者：甘新生[1] 陆仁后[1] 

机构地区：[1]中国科学院水生生物研究所

出　　处：《水生生物学报》1994年第2期107-115,共9页Acta Hydrobiologica Sinica

基　　金：淡水生态与生物技术国家重点实验室课题

摘　　要：草鱼卵巢细胞ＧＣＧ经乙基甲磺酸（ＥＭＳ）诱变，稳定表达３代以后，采取逐步提高培养基中６－疏基嘌呤（６－ＭＰ）的方法，获得对６－ＭＰ稳定抗性的细胞，暂定名ＦＭＲ－１．本文比较了ＧＣＧ细胞及ＦＭＲ－１细胞在含６－ＭＰ（２０μｇ／ｍｌ）和不含６－ＭＰ的培养基中生长曲线的特点，并对上述两种细胞的染色体数目和葡萄糖－６－磷酸脱氢酶电泳图谱进行了比较分析。最后对两种细胞在ＨＡＴ培养基中的生长特点进行了平行对照实验，根据实验结果，可以判定ＦＭＲ－１细胞为草鱼ＨＧＰＲＴ缺陷型细胞。本义还讨论了进一步研究草鱼ＨＧＰＲＴ缺陷型细胞的途径及草鱼ＨＧＰＲＴ缺陷型细胞在鱼类细胞遗传学上的应用前景。The cell line GGG originating from the ovary of grass carp (Ctenpharyngodon idellacur.et val) was treated with ethvl methane sulfonate(EM5)for 72 hours.Aner stable ex-pression of mutation for 3 passages, the eells were treated with 6-mercaptopurine(6-MP)from 4μg/ml to 100μg / ml by stepwise procedures.The isolates did not grow in HAT me-diums. Mutants, which have been named FM R-1,and which are deficient in hypoxanthineguanine phosphoribosyl tranferase(HGPRT) were obtained.Identification of biological characteristics of FMR-1 cells showed that chromosomesdecreased in number compared with those of the parental GCG cells. The growth curves ofFMR-1.ells and GCG cells in TC-199 mediums which contained 6-MP (20μg/ ml)and no6-MP Were also illustrated and compared. The electrophoretogram of G-6-pD isoenzymeof FMR-1.ells displayed almost the same patterns as that of GCG cells.Further measures to study on the fish HGPRT-deficient cells and possible application ofthese cells are discussed.

关 键 词：鱼纲 HGPRT 缺陷型 细胞 诱发变异 

分 类 号：Q959.403[生物学—动物学]