￣（45）Ca跨膜流动测定技术用于中药钙拮抗作用的研究  

STUDYING ON CHINESE HERBAL DRUGS BY USING ￣(45)Ca TRANSMEMBRANE INFLUX TECHNIQUE

作　　者：陈恒留 莫尚武 陈放 刘宁 李文学 金建南 张叔渊 

出　　处：《四川大学学报（自然科学版）》1994年第4期501-507,共7页Journal of Sichuan University(Natural Science Edition)

基　　金：核工业科学基金

摘　　要：应用￣（４５）Ｃａ跨膜流动测定技术研究钙拮抗剂ｖｅｒａｐａｍｉｌ、中药西红花（ＣｒｏｃｕｓＳａｔｉｖｕｓＬ．）、“地奥心血康”（ＤＡＸＸＫ）、川红花（ＣａｒｔｈａｍｕｓｔｉｎｃｔｏｒｉｕｓＬ．）及银杏叶（ＧｉｎｋｇｏｂｉｌｏｂａＬ．）等对大鼠主动脉￣（４５）Ｃａ跨膜内流的作用，以确定其对细胞膜Ｃａ￣（２＋）通道影响。实验结果表明：西红花、川红花、ＤＡＸＸＫ对平滑肌细胞膜上电压依赖Ｃａ￣（２＋）通道（ＰＤＣ）和受体操纵Ｃａ￣（２＋）通道（ＲＯＣ）都有阻滞作用，并以西红花的阻滞作用最强；而银杏叶对细胞膜Ｃａ￣（２＋）通道无阻滞作用。The influences of calcium antagonist-verapamil , Chinese herbal drugs-Crocus sativusL. , Di-ao-xing- xue -kang(DAXXK),Carthamus tinctorius L, and Ginkgo biloba L. leaves onCa￣(2+) transmembrane influx in rat aorta have been studied using￣(45)Ca as a tracer. The results indicatedthat:1. Potenttal-dependent Ca￣(2+) chnnel(PDC)and receptor- operated Ca￣(2+)channel (ROC)in cellmembranes of smooth muscle may be blocked by Crocus sativus L. ,DAXXK and Carthamus tinctoriusL. , and among them Crocus sativus L. appeared inhibitive effcct stronger than others.2. Ginkgo bilobaL. had no effect on Ca￣(2+) channe.3. It is practicable to dertermine whether the plasmalemaCa￣(2+) channel may be blocked by Chinese herbal drugs using radionuclide￣(45)Ca as a tracer.

关 键 词：钙45 跨膜流动测量 钙拮抗剂 

分 类 号：R286.2[医药卫生—中药学]