胶束毛细管电泳测定小野芝麻中毛蕊花糖苷的含量  

MECC Determination of Verbascoside in Galeobdolon chinense (Benth.) C. Y. Wu

作　　者：蒋受军 鲁静[2] 林瑞超[2] 马双成[2] 

机构地区：[1]广西药品检验所,南宁530021 [2]中国药品生物制品检定所,北京100050

出　　处：《药物分析杂志》2005年第2期137-139,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定小野芝麻中毛蕊花糖苷含量的毛细管电泳方法。方法:采用胶束毛细管电泳法(MECC),未涂层弹性石英毛细管(75μm×56 cm,有效长度50 cm);压力进样,压力:138 kPa,进样时间:10 s;电压:15 kV;柱温:25℃;检测波长:350nm;运行缓冲液:50 mmol·L^(-1)硼酸溶液-50 mmol·L^(-1)磷酸二氢钠溶液-20 mmol·L^(-1)脱氧胆酸钠溶液(1 mol·L^(-1)氢氧化钠溶液调pH=8.5);运行时间:35 min。结果:测得平均回收率为96.8%,RSD为1.1%。结论:方法准确可靠,适合于小野芝麻中毛蕊花糖苷的含量测定。Objective: To establish an HPCE method for the determination of verbascoside in Galeobdolon chinense. Methods: The separation was performed on a fused and uncoated silic capillary of 75μn×56cm(50cm of effective length).50 mmol·L^(-1) boric acid-50 mmol·L^(-1) sodium dihydrogen phosphate-20mmol·L^(-1) sodium deoxycholic acid (adjusted pH to 8. 5 with 1 mol·L^(-1) sodium hydroxide)was used as running buffer. Sample were injected hydrodynamically(15 s,138 kPa)into the capillary. The applied voltage was 15 kV and the detection was set at 350 nm. The running time was 35 min . Result:The average recovery was 96. 8% (RSD=1.1%).Conclusion:The method is reliable, and suitable for the determination of verbascoside in Galeobdolon chinense.

关 键 词：胶束毛细管电泳 测定 小野芝麻 毛蕊花糖苷 含量 中药 

分 类 号：R284.1[医药卫生—中药学]