胰腺组织单细胞悬液制备方法的比较研究  

Comparative Study of two Methods for Making Pancreatic Tissue into Single Cell Suspension

作　　者：张召辉[1] 李玺[1] 夏安周[2] 路逵阳[3] 陈复兴[1] 

机构地区：[1]中国人民解放军九七中心医院,江苏徐州221004 [2]徐州医学院药理学教研室,江苏徐州221000 [3]徐州医学院附属医院普通外科,江苏徐州221000

出　　处：《河北医学》2005年第2期143-144,共2页Hebei Medicine

摘　　要：目的:寻找更好的胰腺组织单细胞悬液制备方法。方法:以大鼠冷缺血胰腺组织作为标本,分别采用剪碎和研磨法制备胰腺组织悬液。经台盼蓝染色测细胞存活率,并进行流式细胞术分析。结果:剪碎法细胞形态较完整、存活率高;两种方法细胞凋亡水平(APO)、CV值有显著性差异(P<0.01)。结论:胰腺组织单细胞悬液制备剪碎法优于研磨法。Objective: To explore the mechanical methods for making pancreatic tissue into single cell suspension.Method: All of the samples for this experiment were collected from pancreas of rat with post cold ischemic injury . Single cell suspension got by “cut” and “homogenator” way were detected with FCM(Flow cytometry). The cell viability was assessed by typan blue exclusion;The morphologic changes of cell were observed. Result: Cells got by the method of “cut” were intact,the viability was good,the percentage of APO and CV vales were low. Conclusion: The method of“cut”with sissors for making renal tissue into single cell suspension was better than that of“homogenator”.

关 键 词：单细胞悬液 胰腺 流式细胞术 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]