扩张床吸附直接从牛血中快速分离牛血清白蛋白  

Rapid separation of bovine serum albumin from bovine blood by expanded bed adsorption

作　　者：路秀玲[1] 赵东旭[1] 金业涛[1] 苏志国[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100080

出　　处：《中国生化药物杂志》2005年第1期5-8,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金 (No .2 0 13 60 2 0 )

摘　　要：目的研究扩张床吸附技术直接从牛血中快速分离血浆蛋白。方法用葡萄糖等张液与牛血按4∶1混合,混合液的渗透压在2 80～30 0mOsmo范围内,与血浆的总渗透压相近;pH值降至6 .8,黏度从全血4 .7mPa·s降至1.0mPa·s以下,再用扩张床StreamlineDEAE对血浆蛋白进行吸附洗脱。结果扩张床洗脱液中单体牛血清白蛋白(BSA)的纯度接近70 % ,全血进料时血浆蛋白吸附容量为纯BSA进料的2 1.4 4 %。结论扩张床技术直接从未经固液分离的牛血血浆中分离BSA ,只经一步操作。PurposeTo study a rapid separation of bovine serum albumin (BSA) from bovine blood by expanded bed adsorption(EBA).MethodsThe osmolarity of bovine blood solution was adjusted to 280～300 mOsmo,similar to the osmolarity of the plasma,by mixing the blood with iso-osmolarity glucose solution at a volume ratio of 1∶4. The pH of bovine blood decreased from 7.4 to 6.8,and the viscosity decreased from 4.7mPa·s to 1.0 mPa·s. The dilution was loaded on an expanded bed filled with the media of Streamline DEAE. ResultsHPLC result suggested that the purity of BSA was near to 70% after the elution of the expanded bed adsorption. The capacity of adsorption for plasma protein was 21.44% that of loading the purified BSA.ConclusionRapid and efficient separation of bovine serum albumin from bovine blood solution was achieved by an one-step operation of EBA.

关 键 词：扩张床吸附 牛血清白蛋白 分离 

分 类 号：Q510.3[生物学—生物化学]