紫球藻胞外多糖抗柯萨奇B_3病毒活性研究I  

Antiviral Activity of Extracellular Polysaccharide from Porphyridium sp.Against CVB_3 Virus:Ⅰ

作　　者：刘石生[1] 魏东[1] 王一飞 朱桂芳[1] 李琳[1] 

机构地区：[1]华南理工大学轻工与食品学院 [2]广州暨南生物医药研究开发基地,广州510632

出　　处：《天然产物研究与开发》2005年第1期16-20,共5页Natural Product Research and Development

基　　金：国家自然科学基金项目 (2 0 2 760 2 1);广东省科技计划项目 (2 0 0 3C10 40 2 5 )

摘　　要：要 :采用MTT法研究了紫球藻胞外磺酸化多糖 (ESPS)的细胞毒性 ,在高浓度 (2 5 0 μg/mL)下尚未发现对HEp 2细胞有明显毒性。CPE抑制法发现ESPS能明显抑制细胞的CPE ,在综合、预防、治疗和直接作用四种给药方式下都具有抑制CVB3 的作用。直接作用法的活性最高 ,TI值高达 192 0 6 ,是阳性对照药病毒唑的 31倍。ES PS组分中分子量越大 ,抗病毒活性越高 ,其中分子量大于 30 0k的组分 ,IC50 可低至 0 .75 4 μg/mL ,TI值高达331. 6 ,是病毒唑的 5倍多。结果表明ESPS有强烈的抗CVB3 病毒活性 ,作用机制可能是干扰病毒在细胞表面的吸附及侵染过程。The cytotoxicity of extracellular sulfated polysaccharide(ESPS)from Porphyridium sp.on cultured HEp-2 cell was investigated by MTT method.No obvious cytotoxicity was found at high concentration of ESPS up to 250 μg/mL.The remarkable inhibition effect of ESPS on CPE of cell line was identified by CPE inhibition method.Effect of inhibition on CVB 3 virus can be detected through four drug administration ways,comprehensive,precaution,therapy,and direct-acting.The highest activity was observed with TI value high up to 1920.6 under the direct-acting way,which is 31 times higher than that of the positive control drug,Ribavirin.It is indicated that the higher the molecular weight of ESPS components,the higher the antiviral activity.IC 50 value of ESPS component with MW>300 k dalton can be lowed to 0.754 μg/mL and TI value was high up to 331.6,which is 5 times higher than that of Ribavirin.The results show ESPS has strong antiviral activity against CVB 3 virus,and the mechanism may be related to the interference function of ESPE on the absorption of virus to cell surface and following infection process.

关 键 词：中分子 病毒唑 柯萨奇B3病毒 治疗 抗C 细胞表面 给药方式 组分 磺酸 紫球藻 

分 类 号：Q949.292.1[生物学—植物学] R277.791.6[医药卫生—中医学]