肾缺血再灌注损伤时血清及肾组织中促炎症细胞因子的变化与意义  

Study on Changes and Significance of Proinflammatory Cytokines in Serum and Real Tissues of Rats with Renal Ischemia-reperfusion Injury

作　　者：刘凤[1] 石增立[1] 于小玲[1] 石磊[1] 张树华[1] 董晓青[1] 王宝娃[1] 

机构地区：[1]滨州医学院病理生理学教研室,滨州256603

出　　处：《中国临床医学》2005年第1期174-176,共3页Chinese Journal of Clinical Medicine

摘　　要：目的:探讨促炎症细胞因子白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)在大鼠肾缺血-再灌注损伤(IRI)中的变化和意义。方法:采用夹闭大鼠肾动静脉的方法复制大鼠IRI模型,采用酶联免疫吸附试验(ELISA法)测定肾缺血及再灌注1 h、4 h和24 h时血清和肾组织中IL-6、IL-8和TNF-α含量,并对肾系数、血清尿素氮、肌酐等指标进行检测。结果:单纯缺血组和再灌注4 h、24 h组'肾组织中IL-6水平明显低于假手术组(P<0.05),血清中IL-6水平仅在缺血组低于假手术组;血清与肾组织中的IL-6含量在不同时间内其变化呈直线正相关(r=0.89,P<0.(J5)。肾组织中IL-8、TNF-α水平在单纯缺血和再灌注早期(1 h、4 h组)明显高于假手术组(P<0.01.0.05),随再灌注时间延长两者逐渐下降至对照组水平;血清IL-8在单纯缺血和再灌注1 h组明显低于假手术组(P<0.01),随再灌注时间延长,逐渐接近对照组水平。血清SUN、Scr水平和肾系数在IRI各组均高于对照组。结论:IL-8、TNF-α可能参与了早期肾IRI过程,IL-6在肾IRI中可能起一定保护作用。Objective:To explore the effect of proinflammatory cytokines interleukin-6 (IL-6), IL-8, tumor necrosis factor-α (TNF-α)in rats with renal ischemia-reperfusion injury (IRI). Methods: The model of renal IRI was replicated by closing renal artery and vein in rat and the levels of IL-6, IL-8, TNF-α in renal tissues and serum were determined by enzyme-linked immunosorbent assay (ELISA). The renal coefficients, serum urea nitrogen (SUN) and serum creatinine (SCr) were also measured. Results:The levels of IL-6 in renal tissues in pure ischemia group and ischemia reperfusion 4 (IR4 h) and 24 h hour (IR24 h) groups were lower than that in control group (P<0. 05) ,and the levels of IL-6 in serum only in pure ischemia group was lower than that in control group. Linear positive correlation was found between blood and renal tissues IL-6 concentrations in different stages (P<0. 05). The levels of IL-8,TNF-α in renal tissues in pure ischemia and IR1 h,IR4 h groups were higher than that in control group (P<0. 01 ,0. 05) ,and the levels of IL-8 in serum in pure ischemia and IR1 h groups were lower than that in control group (P<0. 01). The levels of SUN, SCr and renal coefficients in every group of ischemia-reperfusion were higher than that in control group. Conclusion: IL-8, TNF-α might play an important role in early renal IRI. IL-6 might become favourable effect in different stages of renal IRI.

关 键 词：肾组织 IL-6 血清 IL-8 缺血 再灌注 对照组 水平 变化 下降 

分 类 号：R692[医药卫生—泌尿科学] R743[医药卫生—外科学]