复方银杏滴丸对糖皮质激素诱导的脑功能衰退小鼠的保护作用  

Protective effects of compound of Ginkgo biloba extract on cerebral functional decline mice induced by glucocorticoid

作　　者：张爱林[1] 徐秋萍[1] 孙建宁[1] 叶正良[2] 章顺楠[2] 孙鑫[2] 

机构地区：[1]北京中医药大学中药学院药理教研室,北京100102 [2]天津天士力集团有限公司,天津300402

出　　处：《中国新药与临床杂志》2005年第3期177-180,共4页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的: 观察复方银杏滴丸 (CO GBE)对糖皮质激素(GC)诱导的脑功能衰退小鼠的保护作用。方法: 6月龄小鼠隔日皮下注射氢化可的松 30mg·kg-1共计 11次造成脑功能衰退模型,分别采用跳台法和水迷宫法测定小鼠的学习记忆能力,并检测其体重、脾脏指数和胸腺指数。结果:与空白对照组相比,模型小鼠的学习记忆能力明显下降 [跳台试验中,学习能力测定的反应期 (2. 4±s1. 9 )s、潜伏期(41±81)s、停留时间 (13±14 )s、错误次数 (2. 5±2. 4)次。记忆能力测定的潜伏期 (159±144)s、停留时间(3±4)s、错误次数 (0. 6±1. 0)次。水迷宫试验中,学习能力测定的B点到达时间 (61±37)s、错误次数(3. 2±2. 4)次、C点到达时间(65±65)s、错误次数(3. 9±2. 9)次,记忆能力测定的B点到达时间(60±41)s、错误次数(3. 0±1. 0)次,C点到达时间(71±59)s,错误次数 (3. 2±2. 2)次 ];体重明显减轻;脾脏指数减小(P<0.05,P<0. 01);胸腺指数呈减小趋势。CO GBE 15, 30, 60mg·kg-1不同程度地增强模型小鼠的学习记忆能力 (P<0.05,P<0. 01), 60mg·kg-1还可以明显抑制模型小鼠体重下降,P<0. 05,增加其脾脏指数 (98±33 )mg·10g-1体重,P<0. 05,未见药物对模型动物胸腺指数有影响。结论:COAIM: To observe the protective effects of compound of Ginkgo biloba extract on cerebral functional decline mice induced by glucocorticoid. METHODS:The mice of 6 mo were injected subcutaneously of hydrocortisone 30 mg·kg -1 every other day, 11 times totally, to create cerebral functional decline model. Jumping stand and water maze test were adopted to estimate the learning and memory abilities of mice. The body weight, spleen index and thymus index were also assayed. RESULTS: Compared with the control group, the learning and memory abilities of the model mice declined significantly. In jumping stand test, the period for reflect, latency, stagnant period, and error times were (2.4±1.9)s, (41±81)s, (13±14)s , (2.5±2.4) respectively. In memory ability assay, the latency, stagnant period, and error times were (159±144)s, (3±4)s, (0.6±1.0) respectively. In water maze test, times for mice arriving to point B were (61±37)s, and error times were (3.2± 2.4), to point C were (65±65)s and error times (3.9± 2.9) respectively during learning ability assays. The data for up-grading were (60±41)s, (3.0± 1.0), (71±59)s and (3.2±2.2) in memory ability assay, P<0.05, P<0.01. Body weight lost in model mice(37±4)g and spleen index decreased ((66± 31) mg·10 g -1 body weight). Thymus index only showed decreasing trend. All three compounds of Ginkgo biloba extract doses of 15, 30, 60 mg·kg -1 could improve the learning and memory abilities of model mice to different degrees (P<0.05, P< 0.01). 60 mg· kg -1 could also restrain the decrease of body weight ((40±3)g, P<0.05) and increase spleen index ((98±33) mg·10 g -1 body weight, P< 0.05 ). The effect of the prescription on the thymus index of model mice was not shown in this study. CONCLUSION: Compound of Ginkgo biloba extract can ameliorate the learning and memory abilities of cerebral functional decline mice induced by glucocorticoid obviously. The highest dose can impede the immunosuppressive action and

关 键 词：氢化可的松 小鼠 学习 记忆 复方银杏滴丸 

分 类 号：R285.5[医药卫生—中药学] R971[医药卫生—中医学]