两种膜截留分子量超滤及醇沉对清热解毒口服液纯化效果的比较  

Comparison of Purification Effects of Qingrejiedukoufuye (Heat - clearing and Detoxicating Oral Liquid) by Methods of Ultra - filtration and Alcohol Precipitation of Retain Molecular Weight of Two Membranes

作　　者：李淑莉[1] 刘振丽[1] 宋志前[1] 付俊[1] 

机构地区：[1]中国中医研究院基础理论研究所,北京100700

出　　处：《世界科学技术-中医药现代化》2005年第1期52-55,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家中医药管理局课题:超滤膜截留分子量与超滤效果关系的研究(编号:0203ZP63)

摘　　要：目的:考察超滤膜截留分子量对纯化效果的影响,并与醇沉法进行比较。方法:采用超滤法(膜截留分子量20000、70000,称之20000膜、70000膜,下同)和乙醇沉淀法纯化清热解毒口服液。以黄芩中黄芩苷、金银花中绿原酸保留率、杂质多糖和鞣质的清除率以及膜通量变化为指标, 比较两种膜超滤效果,并与乙醇沉淀法比较。结果:黄芩苷、绿原酸保留率两种截留分子量的膜相差不大,但明显高于醇沉;去除多糖以20000膜最好,其次为70000膜,醇沉最差;去除鞣质以70000 膜最好,其次为醇沉,20000膜最差;70000膜膜通量衰减小于20000膜,超滤等量药液所需时间缩短两倍。结论:选择适宜的超滤膜截留分子量可以达到纯化中药的效果。Objective: To investigate the impact of retain molecular weight of milipore filters on the effect of purification and compare it with the effect of purification made by the method of alcohol precipitation. Method: To purify Qingre-jiedukoufuye (Heat - clearing and Detoxicating Oral Liquid) by the method of ultra -filtration (retain molecular weights 20000 and 70000 of membranes) and the method of alcohol preciptiation. To compare the effect of ultra - filtration of the said two membranes by the way of taking for indexes the retention rate of baicalin in Radix Scutellariae and that of chrologenic acid and the clearance rate of impure polysaccharides and tannin of Flos Lonicerae as well as the change of membrane flux and compare it with the effect of alcohol precipitation. Result: For the retention rate of baicalin and chrologenic acid the difference of purification effect of the membranes with two retain molecular weights is small but notably larger than that from the method of alcohol precipitation; for the clearance rate of polysaccharides the purification effect from the membrane with 20000 of retain molecular weight is the best and then that from the membrane with 70000, while the purification effect from the method of alcohol precipitation is the worse; for the clearance rate of tannin the purification effect from the membrane with 70000 of retain molecular weight is the best and then that from the method of alcohol precipitation, while the purification effect from the membrane with 20000 falls worse; the attenuation rate of flux of the membrane with 70000 of retain molecular weight is lower than of the membrane with 20000 and the time needed for equivalent liquid medicine by the method of ultra - filtration reduces twice. Conclusion: The purification effects of Chinese drugs can be achieved by selecting appropriate retain molecular weight of milipore filters.

关 键 词：乙醇沉淀法 清热解毒口服液 黄芩苷 鞣质 绿原酸 纯化效果 醇沉法 超滤膜 去除 膜通量 

分 类 号：N1[自然科学总论] R284[医药卫生—中药学]