三价转基因抗病毒西瓜的培育  

Creation of Trivalent Transgenic Watermelon Resistant to Virus Infection

作　　者：牛胜鸟[1] 黄学森 王锡民[3] 于嘉林[1] 赵福兴 李大伟[1] 王生有 翟光明 师范生 

机构地区：[1]中国农业大学农业生物技术国家重点实验室,北京100094 [2]山西金鼎生物种业股份有限公司,运城044000 [3]新加坡国立大学,新加坡117543

出　　处：《农业生物技术学报》2005年第1期10-15,共6页Journal of Agricultural Biotechnology

摘　　要：利用西瓜花叶病毒(Watermelonmosaicvirus,WMV)外壳蛋白(CP)基因、西葫芦黄花叶病毒(Zucchiniyellowmosaicvirus,ZYMV)复制酶(NIb)基因和黄瓜花叶病毒(Cucumbermosaicvirus,CMV)复制酶基因构建三价植物表达载体,根癌农杆菌(Agrobacteriumtumefaciens)介导法转化西瓜(Citrulluslanatus)子叶外植体获得再生植株。经PCR和Southern-blot检测,证明目的基因成功地导入西瓜植株,并能够在后代植株中遗传,转化效率约为1.7‰。在温室和大田对T2、T3代转基因株系进行了病毒接种实验和抗病性筛选鉴定,转基因西瓜株系表现出感病、抗病、免疫和症状恢复等不同的类型。其中BH1-7株系的T3代植株对ZYMV和WMV的抗病能力普遍达到中等水平以上,并保持了受体西瓜品系原有的优良农艺性状。A trivalent vector containing the genes of Watermelon mosaic virus (WMV) coat protein (CP), replicases of Zucchini yellow mosaic virus (ZYMV) and Cucumber mosaic virus (CMV) was constructed for transformation of the watermelon (Citrullus lanatus) plants mediated by Agrobacterium tumefaciens. The foreign genes integrated into the regenerated progenies were identified by PCR and Southern-blot. The transformation efficiency was about 1.7‰. By mechanical inoculation, the detections of resistance to virus infection were carried out in the greenhouse and field trial. The transgenic watermelons of the different lines represented different phenotypes of susceptible, resistant, immune and recovery from the virus infections in the late stage, among which the resistance showed by T3 plants of BH1-7 line was in a relative high level. This result indicated it is possible to create new varieties of watermelon resistant to virus infection by transgenic protocol.

关 键 词：西瓜 株系 植株 转基因 培育 黄花叶病毒 子叶外植体 抗病毒 CP 受体 

分 类 号：S651[农业科学—果树学] S435[农业科学—园艺学]