RP-HPLC法测定不同来源连翘药材中连翘苷的含量  

Determination of Phillyrin in Fructus Forsythiae from Different Places by RP-HPLC

作　　者：侯莉[1] 王强[1] 任晋斌 

机构地区：[1]中国药科大学,江苏南京210038 [2]山西省药品检验所,山西太原030001

出　　处：《现代中药研究与实践》2005年第1期48-50,共3页Research and Practice on Chinese Medicines

摘　　要：目的　建立连翘药材中连翘苷的含量测定方法。方法　采用ZORBAXSB C18柱 ,乙腈 水 (2 3∶77)为流动相 ,流速 1mL/min ,检测波长 2 2 9nm。结果　在选定的色谱条件下 ,连翘苷分离度良好 ,线性好 ,线性范围宽 ,平均回收率 98.8% ,RSD =3.1%。结论　此法简便 ,灵敏 ,适用于连翘药材中连翘苷含量测定 。Objective To develop a method for the determination of phillyrin in Fructus Forsythiae purchased from different places.Method HPLC was achieved with a ZORBAX SB-C 18 analysis column, the mobile phase was acetonitrile-water(23∶77), detection wavelength was 229 nm,flow rate was 1.0 mL/min.Result The method was linear at the range of 0.125~1.25 μg,r=0.999 7, the average recovery was 98.8%,RSD was 3.1%.Conclusion This method is simple,sensitive, and can be used for the daily analysis of phillyrin in Fructus Forsythiae,and the result showes the content of phillyrin in Fructus Forsythiae purchased from different places varies remarkably.

关 键 词：连翘苷 RP-HPLC法 含量测定方法 药材 色谱条件 RSD 测定结果 C18柱 平均回收率 来源 

分 类 号：R284[医药卫生—中药学] R286.0[医药卫生—中医学]