RP-HPLC法测定灌胃赤芍胡椒复方小鼠血浆中芍药苷的含量  

Determination of paeoniflorin in mouse plasma after ig compound decoction of Radix Paeoniae Rubra with Fructus Piperis by RP-HPLC

作　　者：裴瑾[1] 杨祖贻[1] 刘荣敏[1] 程佳[1] 万德光[2] 胡荣[2] 

机构地区：[1]四川省肿瘤研究所,四川成都610041 [2]成都中医药大学药学院,四川成都610075

出　　处：《中草药》2005年第3期358-360,共3页Chinese Traditional and Herbal Drugs

基　　金：国家中医药管理局科研基金资助项目 (0 2 -0 3 ZP5 0 ) ;四川省中医药管理局科研基金资助项目 (2 0 0 2 17)

摘　　要：目的　建立小鼠口服赤芍胡椒复方后血浆中芍药苷浓度的RP- HPL C定量分析方法。方法　以甲醇-水(38∶6 2 )为流动相。色谱柱为Krom asil C1 8(2 5 0 mm×4 .6 mm ,7μm ) ,体积流量为0 .5 m L / m in,以二极管阵列检测器获取2 30 nm波长下数据,采用外标定量法。结果　芍药苷与血浆中其他成分能很好分离,芍药苷在5 .0～2 5 0 .0ng/μL线性关系良好(r=0 .9999) ,最低检测质量浓度为1.4 9ng/μL ,平均回收率大于90 %。结论　本方法准确性、灵敏度、专一性高,可为芍药苷的体内血浆药物浓度分析提供有意义的方法学参考。Objective An HPLC method was established for determination of paeoniflorin in plasma after ig compound decoction of Radix Paeoniae Rubra with Fructus Piperis to mice. Methods The conditions of chromatography: Kromasil C 18 column (250 mm × 4.6 mm, 7 μm) was used with a mobile phase of CH 3OH-H 2O ( 38∶ 62); flow rate was 0.5 mL/min; the detecting wavelength was 230 nm; external standard method was quantitative analysis method. Results Paeoniflorin was fully separated from the other components in plasma. The linear range was 5.0—250.0 ng/μL (r= 0.999 9 ), the lowest detectability was 1.49 ng/μL, the average recovery was higher than 90%. Conclusion This method specially provides an accurate and sensitive way in detecting the in vivo blood concentration of paeoniflorin in plasma.

关 键 词：赤芍胡椒复方 芍药苷 高效液相色谱 血药浓度 

分 类 号：R286.02[医药卫生—中药学]