提取工艺对脑得生抗大鼠局灶性脑梗死的影响  

Effects of different extracting techniques on Naodesheng against focal cerebral infarction of rats

作　　者：欧来良[1] 李家政[2] 王瑞芳[1] 史作清[1] 施荣富[1] 

机构地区：[1]南开大学生物活性材料教育部重点实验室,分子生物学研究所,天津300071 [2]天津大学高分子材料研究所,天津300072

出　　处：《中草药》2005年第3期393-395,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目 (2 99740 15 )

摘　　要：目的　通过对脑得生不同提取工艺——分煎与合煎抗大鼠局灶性脑梗死影响的实验研究,探索中药复方分煎工艺在中药现代化进程中实施的可能性。方法　利用Fe Cl3损伤血管制作大鼠大脑中动脉血栓模型,于血栓形成后5 d内,给药组连续ig脑得生按药典法制备制剂(N 1,1.2 g/ kg)、脑得生各单味药分煎,吸附树脂富集、分离有效部位混合制剂(N2 ,0 .2 g/ kg)和脑得生各单味药合煎,吸附树脂富集、分离,有效部位混合制剂(N3,0 .6 g/kg) ,观察大鼠行为评分、脑梗死面积及病理组织学变化。结果　经N 1、N 2和N 3治疗的大脑中动脉血栓模型大鼠的行为评分、脑组织梗死面积与模型组相比差异均有显著性(P<0 .0 1) ,组织病理变化较模型组也有明显改善。而N1、N2和N3组间相比差异无统计学意义。结论　采用单味药有效成分或部位分别提取,而后组方所得复方制剂,并不会降低原方的整体疗效发挥。该研究结果对其他复方研究也具有一定的推广价值。Objective To investigate the feasibility of the individual decocting and codecocting techniques for compound herbal medicine in the course of Chinese materia medica (CMM) modernization through studies on the effect of Naodesheng various preparations on the focal cerebral infarction of rats. Methods The cerebral artery thrombosis model was made by FeCl 3-induced damage to local cerebral middle artery of rats. Within five days since thrombogenesis, the behavioral score of neurological deficit was evaluated and the size of cerebral infarction was measured after the rats were fed by continuously ig Naodesheng Ⅰ (N1, 1.2 g/kg), prepared according to Pharmacopeia, Naodesheny Ⅱ (N2, 0.2 g/kg) prepared by decocting single herbs respectively, then mixing their fractions together, and Nadeshang Ⅲ (N3, 0.6 g/kg) prepared by codecocting herbs together, then isolating their effective ingredients. Also the histopathological changes were examined. Results Both the behavioral score of neurological deficit and the size of cerebral infarction in the treatment groups were significantly diminished as compared to the control group (P<0.01) and histopathological group showed significant changes compared to the model group. While among the N1, N2, and N3 groups there was no statistically significance in terms of the above-mentioned parameters. Conclusion The results demonstrate that the curative effect of the individual decocting technique of compound herbal medicine is not difference from that of codecocting technique. Therefore, the individual extraction technique could be applied to compound CMM in order to reduce the difficulty in extraction, separation, analysis and so on.

关 键 词：脑得生 提取工艺 脑梗死 

分 类 号：R285.5[医药卫生—中药学]