丁雌核发育鱼的异源精子诱导及其与亲本的RAPD比较分析  

The inducing of gynogenetic tench by heterologous sperm and comparison with parents by RAPD

作　　者：凌去非[1] 李思发[1] 张海军[2] 殷建国[2] 

机构地区：[1]上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090 [2]新疆生产建设兵团农十师额河特种鱼类繁育场,新疆北屯836000

出　　处：《水产学报》2005年第1期120-123,共4页Journal of Fisheries of China

基　　金：新疆生产建设兵团科委农业科学研究与技术开发项目(NKB02N10NK16XM)

摘　　要：Gynogenesis is thought to be a useful method to generate fully inbred line and to initiate monosex culture in teleost fish. This article presents results of a study of gynogenesis of tench, Tinca tinca L. from Eltrix River of Xinjiang, China, induced by the common carp (Cyprinus carpio) sperm inactivated with UV irradiation. When cold shocking method was used to prevent extrusion of the second polar body in order to produce gynogenetic diploids, the optimum treated parameters were screened as cold shocking for 20min at 4℃, 5min after insemination. On this condition, 10.23% of the treated eggs survived at feeding stage. A comparative study between the gynogenesis and their parents was made by RAPD technique. 82 random primers of 10 nucleotide long sequence were used in RAPD analysis. The results showed that each primer gives (3-12) fragments for each sample with the fragment length (330-2 460) bp, and the genetic similarity between gynogenesis and their mother was 97.4%, while the genetic similarity between gynogenesis and their father was 27.4%, and there were no bands only shared by gynogenesis and their father, the common carp. It revealed that the chromosome of their father, the common carp, did not penetrate into the construction of gynogenetic tench.Gynogenesis is thought to be a useful method to generate fully inbred line and to initiate monosex culture in teleost fish. This article presents results of a study of gynogenesis of tench, Tinca tinca L. from Eltrix River of Xinjiang, China, induced by the common carp (Cyprinus carpio) sperm inactivated with UV irradiation. When cold shocking method was used to prevent extrusion of the second polar body in order to produce gynogenetic diploids, the optimum treated parameters were screened as cold shocking for 20min at 4℃, 5min after insemination. On this condition, 10.23% of the treated eggs survived at feeding stage. A comparative study between the gynogenesis and their parents was made by RAPD technique. 82 random primers of 10 nucleotide long sequence were used in RAPD analysis. The results showed that each primer gives (3-12) fragments for each sample with the fragment length (330-2 460) bp, and the genetic similarity between gynogenesis and their mother was 97.4%, while the genetic similarity between gynogenesis and their father was 27.4%, and there were no bands only shared by gynogenesis and their father, the common carp. It revealed that the chromosome of their father, the common carp, did not penetrate into the construction of gynogenetic tench.

关 键 词：丁鱥 雌核发育 随机扩增多态DNA 

分 类 号：S917[农业科学—水产科学]