傅里叶变换红外光谱应用于乳腺癌临床诊断的探索  

FTIR Spectroscopic Explorations of Clinical Practice of Breast Cancer

作　　者：凌晓锋[1] 徐智[1] 徐怡庄[2] 李庆波[2] 周苏[1] 张莉[2] 赵红梅[1] 侯纯升[1] 王立新[1] 侯宽永[1] 周孝思[1] 吴瑾光[2] 

机构地区：[1]北京大学第三医院普通外科,北京100083 [2]北京大学化学学院,北京100871

出　　处：《光谱学与光谱分析》2005年第2期198-200,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金重点项目(50203001;30371604);科技部"973"前期专项(2002CCA01900);教育部博士点基金资助

摘　　要：应用了ATR探头测定了10例正常乳腺组织块和8例乳腺癌组织块的傅里叶变换红外(FTIR)光谱,用统计学的方法详细比较了乳腺癌组织与正常乳腺组织的13条谱带的19个红外光谱指标的差异。结果表明乳腺癌组织的红外光谱中与蛋白质、脂类、碳水化合物和核酸相关的谱带同正常乳腺组织之间存在明显的差异(1)N—H的相对强度明显增高,蛋白质酰胺Ⅰ带显著红移。(2)—CH2基团的对称伸缩振动和反对称伸缩振动以及CO的伸缩振动明显减少;(—CH2)n变角振动的相对强度降低。(3)1160cm-1谱带与1120cm-1谱带的相对强度明显降低。(4)1080cm-1谱带红移。这些差异是应用红外光谱诊断乳腺癌的基础。FTIR光谱在乳腺癌的临床诊断和治疗方面有着十分美好的前景。The authors detected ten normal breast tissue samples and eight breast cancer samples by FTIR spectroscopy with an ATR probe. Nineteen variables of thirteen bands in the spectra were compared using standard statistic methods. The results demonstrated that bands of protein, lipid, carbohydrate, and nucleic acid from cancerous samples were significantly different from those from normal ones: (1) The relative intensity of N-H band increased and amide I band shifted to lower wave number significantly; (2)Symmetric and antisyrnmetric vibrations of -CH2 group, C = O vibration, and relative intensity of (-CH2), decreased; (3)The intensity of 1 160 cm(-1) band was much weaker than that of 1 120 cm(-1);(4) The band of P = O or P-O-C shifted toward lower wave number. The authors believe that FTIR spectroscopy has a promising future in breast cancer diagnosis.

关 键 词：乳腺癌组织 正常乳腺组织 临床诊断 CH 治疗方 增高 脂类 谱带 相对强度 红外光谱 

分 类 号：O561.3[理学—原子与分子物理] R737.9[理学—物理]