树舌多糖GF注射液对HepA荷瘤鼠T细胞亚群、白介素-2及巨噬细胞吞噬功能的影响  

Effects of Ganoderma Appanatum Polysaccharides GF Injection on T cell Subpopulations,IL-2 and Phagocytic Function of Macrophage in HepA-bearing Mice

作　　者：管宇[1] 于英君[1] 

机构地区：[1]黑龙江中医药大学生物化学及分子生物学教研室,黑龙江哈尔滨150040

出　　处：《中国药业》2005年第3期31-33,共3页China Pharmaceuticals

基　　金：黑龙江省科技攻关项目;项目编号:G00C 200902。

摘　　要：目的:探讨研制中的抗肿瘤新药树舌多糖GF注射液(GFi)对荷瘤小鼠外周血T细胞亚群、血清白介素-2(IL-2)及巨噬细胞(MΦ)吞噬功能的影响。方法:HepA 荷瘤鼠动物模型,以流式细胞术测定外周血T细胞亚群功能,ELISA 法测血清IL-2,以腹腔注射鸡红细胞测定MΦ吞噬功能。结果:GFi能够使荷瘤小鼠外周血CD4+ ,CD8+T细胞数量增加(P<0.01) ,调节CD4+/CD8+比例;提高血清IL-2的水平(P<0.05);并显著提高荷瘤小鼠腹腔MΦ的吞噬率和吞噬指数(P<0.01)。结论:GFi对肿瘤机体免疫功能有明显的促进作用。Objective:To research the immunological function of GFi on T cell subpopulations,IL-2 and phagocytic power of belly cavity macrophage in HepA-bearing mice.Methods:The animal model of HepA-loading mice were established;the number of T lymphocyte subsets in peripheral blood were measured by McAb indirect immunofluorescence technique;the content of IL-2 in serum were tested by ELISA;the phagocytic power were observed by injecting CRBC into mice belly cavity.Results:GFi could significantly augment the number of CD4+ and CD8+T cell in peripheral blood(P < 0.01),restore the proportion of CD4+/CD8+;increase the level of IL-2 in serum(P < 0.05) and enhance abdominal cavity macrophage phagecytic power of tumor-bearing mice.Conclusion:GFi could remarkably improve immunological function of HepA-bearing mice.

关 键 词：树舌多糖 肿瘤 免疫功能 T细胞亚群 IL-2 吞噬细胞(MΦ) 酶联免疫吸附法 流式细胞术 

分 类 号：R967[医药卫生—药理学] R979.11[医药卫生—药学]