比色法测定黄蜀葵花总黄酮的含量  

Colorimetric determination of the total content of the flavonoids in Abelmoschus manihot

作　　者：崔燕[1] 卢志强[1] 景瑞[1] 赵春荣[1] 

机构地区：[1]山东省生物药物研究院,山东济南250108

出　　处：《食品与药品》2005年第02A期49-51,共3页Food and Drug

摘　　要：目的　采用紫外分光光度法测定黄蜀葵花中总黄酮的含量。方法　用索氏提取法 ,以芦丁为对照品 ,以亚硝酸钠 硝酸铝 氢氧化钠为显色剂 ,检测波长为 5 0 0nm。结果　线性范围为 0 .0 12～ 0 .0 72mg ml,平均回收率为 99.72 % ,相对标准偏差(RSD)为 2 .6 2 %。结论　本法为控制黄蜀葵花的内在质量提供了依据。Objective To analyze the total content of flavonoids in Abelmoschus manihot by colorimetric method.Method Abelmoschus manihot was distilled with Soxhlet extraction process,rutin was used as reference substance and the detecting wavelength was 500nm.Result The linearity of flavonoids was fine in 0.012～0.072mg/ml,average recovery rate 99.72%, RSD 2.62%( n =5).Conclusion The method has laid the foundation for the control of the inherent quality in Abelmoschus manihot.

关 键 词：比色法 黄蜀葵花 索氏提取 黄酮 

分 类 号：S682.162[农业科学—观赏园艺]