秋水仙素处理兰花原球茎对其生长和诱变效应的影响  

EFFECTS OF COLCHICINE TREATMENT ON GROWTH, DIFFERENTIATION AND MUTAGENSEIS OF PROTOCORM-LIKE-BODY (PLB) OF ORCHID

作　　者：张志胜[1] 谢利[1] 萧爱兴[1] 何琼英[1] 姜蕾[1] 

机构地区：[1]华南农业大学农学院,广东广州510642

出　　处：《核农学报》2005年第1期19-23,共5页Journal of Nuclear Agricultural Sciences

基　　金：广东省"九五"重点攻关课题 (962 2 0 3 3 10 ) ;横向联合课题 (4 10 0 H0 2 0 5 2 )

摘　　要：本研究表明 ,秋水仙素处理对原球茎生长和分化起抑制作用 ;浓度越高 ,抑制作用越强 ,处理时间不同 ,抑制作用也不同。秋水仙素处理后的再生植株群体中 ,平均变异植株的百分率为 9 0 6% ,是对照材料的 3 7倍。变异植株的类型包括植株粗壮、多叶、叶片变宽、变厚、叶片墨绿色、矮化、线艺和叶艺等。处理浓度为 0 1 0 %、时间为 48h时 ,植株变异率最高。The effects of colchicine treatment on the growth, differentiation and mutagenesis of orchid PLBs were studied. The results showed that colchicine treatment had inhibition effects on the growth and differentiation of PLBs, and the higher concentration colchicine was, the stronger the inhibition effect was. Different treatment time caused the different effects. Regenerated plants were found mutant in sturdy, variegation, dark color and so on. The ratio of variants regenerated from PLBs treated with colchicine was obviously higher than that of control. The high variants happened in treatment of 0.10% colchicine and 48h.=

关 键 词：秋水仙素 植株 原球茎 生长 兰花 诱变效应 矮化 处理 抑制作用 对照 

分 类 号：S682.3[农业科学—观赏园艺]