芽孢杆菌原生质体的形成、再生及种间融合的研究  

STUDIES ON FORMATION, REGENERATION AND INTERSPECIFIC FUSION OF PROTOPLASTS FROM BACILLUS SUBTILIS AND BACILLUS THURINGIENSIS

作　　者：刘伊强 王雅平[1] 潘乃穟 陈章良[1] 

机构地区：[1]北京大学生物系,北京100871

出　　处：《微生物学报》1994年第1期76-80,共5页Acta Microbiologica Sinica

摘　　要：原生质体融合技术不仅能使遗传基因高频率重组,而且可以集双亲优良遗传性状为一体.自Schaeffer等人成功地进行了微生物原生质体融合以来,这项技术便广为人们所接受.枯草芽孢杆菌分泌的抗菌蛋白能抑制多种植物病原菌的生长,苏云金芽孢杆菌生成的伴孢晶体蛋白可毒杀植物害虫.利用原生质体融合技术将这两种芽孢杆菌抑菌杀虫的遗传特性融为一体,选育出防治植物病虫害的新一代生防菌株成为可能,有关这方面的研究国内外尚未见报道.本文报道了抗菌蛋白产生菌TG26-10和晶体蛋白产生菌AS1.904-17原生质体的形成,再生及种间融合的影响因素,为进一步筛选目的融合子提供基础.Protoplasts of two doubly auxtrophic strains, Bacillus subtilis TG26-10 producing antifungal proteins and Bacillus thuringiensis AS 1. 904-17 producing crystal proteins, were fused by polyethylene glycol treatment. The optimum conditions for protoplast formation, regeneration and fusion were found. Under these conditions, the rates of protoplast formation were 94.2% and 93.9%, respectively , the rates of protoplast regeneration were 18.26% and 8.63% respectively and the rate of interspecific fusion was 7. 52 × 10 6. The obtained fusants were subcultived on MM medium plates at least 10 times and 19. 5% of them were stable. There were apparent differences between parental strains and fusants in colony and cell morphology.

关 键 词：芽孢杆菌属 原生质体 形成 再生 

分 类 号：Q939.110.5[生物学—微生物学]