携带SHV类超广谱β-内酰胺酶的吉戈菲肠杆菌北京株的研究  

A PLASMID-MEDIATED SHV TYPE EXTENDED-SPECTRUM β-LACTAMASE IN BEIJING ISOLATE OF ENTEROBACTER GERGOVIAE

作　　者：程玉林[1] 李银太[2] 陈民钧[1] 

机构地区：[1]中国医学科学院北京协和医院,北京100730 [2]军事医学科学院五所

出　　处：《微生物学报》1994年第2期106-112,共7页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助项目

摘　　要：用接合转移的遗传学方法证实了临床分离菌株吉戈菲肠杆菌(Enterobactergergoviae)3773含有一约60kb的可转移质粒,又用测耐药表型、酶水解率及基因片段杂交等方法证实了该质粒上有一编码产生超广谱β-内酰胺酶(ESbla)的基因.其大肠杆菌接合子除了头霉甲氧塞吩(cefoxitin)和亚胺硫霉素(imipenem)外,几乎对所有测定的β-内酰胺类药物都表现耐药,β-内酰胺酸抑制剂——棒酸(clavulanate)可抑制此ESbla的活性.携带此ESbla基因的质粒的一片段可与SHV-1的一结构基因片段杂交,说明此酶是SHV类的ESbla.By conjugation experiment, an extended-spectrum β-lactamase (ESbla)encod-ed on a transferable plasmid of-60 Kilobases was found in a clinical strain of Enterobac-ter gergoviae isolated from Peking Union Medical College Hospital. Its E. coli transcon-jugant was resistant to all β-lactams tested except cefoxitin and imipenem. Clavulanic acid, a classical β-lactamase inhibitor,inhibited its activity. Hybridization with an intra-genic probe for SHV-1 revealed that this ESbla may be related to,or derived from SHV enzyme.

关 键 词：吉戈菲肠杆菌 SHV类 Β-内酰胺酶 

分 类 号：R378.2[医药卫生—病原生物学]