吉戈菲肠杆菌中SHV-2耐药基因的序列分析  

NUCLEOTIDE SEQUENCE ANALYSIS OF SHV-2 β-LACTAMASE GENE IN ENTEROBACTER GERGOVIAE

作　　者：程玉林[1] 陈民钧[1] 

机构地区：[1]中国医学科学院北京协和医院,北京100730

出　　处：《微生物学报》1994年第3期226-230,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助项目

摘　　要：近来一个值得注意的现象,即肠杆菌科中某些菌属对头孢噻甲羧肟(ceftazidime,caz)等三代头孢菌素的耐药有不同程度增长,这是由于细菌产生了超广谱β-内酰胺酶(ESbla),或染色体Ⅰ型酶所致.在我们前一步的研究中,发现一耐caz的Ent.gergoviae菌株,含有一约60kb的可接合转移质粒pC3773.其上有一编码SHV类ESbla的基因,为深入了解此ESbla基因的分子特点,以及酶结构与功能的关系,本文用测定核苷酸序列的方法进行了研究.The extended-spectrum β-lactamase (ESbla) gene on R-plasmid harbored in E. coli C3773,a transconjugant of the clinical isolate of Ent. gergoviae 3773 from our hospital, was cloned and two fragments were subcloned into M13. The nucleotide sequences of two fragments were determined. The primary structure of this ESbla deduced from nucleotide sequence had only a Gly→Ser change at position 234 in comparison with SHV-1, which was supposed to be responsible for extended-spectrum activity and resistance to 3rd generation cephalosporins. The result suggested that the ESbla appearing in our hospital was SHV-2.

关 键 词：吉戈菲肠杆菌 抗药性 基因 序列 

分 类 号：Q939.110.3[生物学—微生物学]