Frankia与链霉菌融合子特性的研究  

STUDY OF THE CHARACTERISTICS OF THE FUSANTS FUSED BY FRANKIA STRAIN Cc01 AND STREPTOMYCES AUREUS STRAIN GL

作　　者：邹铧[1] 弥铁镌 张忠泽[1] 苏凤岩[1] 郝东梅[3] 王育英[1] 丁鉴[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110015 [2]沈阳医学院生化教研室,沈阳110031 [3]中国医科大学分子遗传室,沈阳110001

出　　处：《微生物学报》1994年第4期310-315,共6页Acta Microbiologica Sinica

摘　　要：将Frankia菌株CcOl与金色链霉菌GL原生质体融合,得到3株融合子,均具有GL生长快的特性与CcOl的结瘤固氮能力.固体培养时,3株融合子呈现出与GL不同的颜色;且均具有Frankia菌的顶囊形态,以及链霉菌的链状孢子丝结构.与两亲本相同,3株融合子均对大肠杆菌有抗性,其中F4与GL的抗菌谱基本相同.在传10代之后,它们仍具有结瘤与固氮能力.血清学分析表明,F1与F6兼具两亲本的特异抗原,而F4仅具有GL的特异抗原,融合子F1、F6较F4在遗传上更为稳定.Three fusants were obtained by fusing the protoplasts of Frankia strain Cc01 and Streptomyces aureus strain GL. All three fusants grew fast like GL, and could nodulate and fix nitrogen like that of strain CcOl. The colors of the hypha of the three fusants were different from their parent strain GL when they grew in agar media. All three fusants had vesicles which was the typical shape of Frankia strains, and chian spores like strain GL. They all had the antibacterial action to E. coli as their parents. Moreover, the antibiogram of fusant F4 was almost the same as its parent strain GL. Being transfered more than 10 times in the agar medium, all three fusants still had nodulation and nitrogen fixation abilities, but less than their parent Cc01. The serulogical analysis indicated that fusants F1 and F6 had both parents characteristic antigens, while fusant F4 had only the GL characteristic antigens. This suggested that fusants F1 and F6 were more stable in genetics and more believable.

关 键 词：链霉菌 融合子 法兰克氏菌属 

分 类 号：Q939.130.5[生物学—微生物学]