人免疫缺陷病毒I型抗AZT抗药株的体外研究  

Study of HIV-1 Resistant to AZT Virus Strain in Vitro

作　　者：董关木[1] Guido.,A 

机构地区：[1]中国药品生物制品检定所

出　　处：《微生物学免疫学进展》1994年第4期4-7,共4页Progress In Microbiology and Immunology

摘　　要：人免疫缺陷病毒Ⅰ型（ＨＩＶ－１）抗３＇─叠氮─３＇─脱氧胸腺嘧啶（ＡＺＴ）抗药株经体外感染Ｃ８１６６淋巴细胞在高浓度ＡＺＴ条件下筛选获得，并暂命名为ＨＩＶ─１─Ｒ株。该抗药株与ＨＴＬＶ─ⅢＢ株相比，在同一感染复数（Ｍ０１）病毒量感染Ｃ８１６６细胞，经不同浓度的ＡＺＴ处理后，其复制的病毒量和对ＡＺＴ的敏感性有显著的差异，抗药株感染Ｃ８１６６细胞，加ＡＺＴ处理后，分离细胞ＤＮＡ作ＰＣＲ扩增后分析，特异性病毒的ＤＮＡ量比敏感株高１００倍以上，显示其抗药性作用点在病毒逆转录ＤＮＡ前。3'-Azido-3'-deoxythmidine (AZT) resistant strain of human immunodeficiency virus type-1(HIV-1)was obtained by growing HTLV-Ⅲ B strain in C8166 cells culture in the presence of inhibitory concentration of AZT. Resistant'strain revealed that infectious virus yield and degrees of sensitivity to AZT were drastic different from HTLV-ⅢB during propagation in C8166 cells in the presence of the drug.PCR experiments performed on DNA extracted from C8166 cells infected with a resistant revealed that viral DNA was produced in presence of inhibitory concentration of AZT. It was revealed high resistance drug character.

关 键 词：AZT 叠氮 脱氧胸腺嘧啶 艾滋病毒 抗药株 

分 类 号：R373.9[医药卫生—病原生物学] R978.7[医药卫生—基础医学]