乙肝阳性血浆中HBsAg的滴度与保存温度和疫苗收量关系  

The Relationships Among HBsAg Titer of Positive HBsAg Plasma and Temperature of Storage and Yield of HB-Vaccine

作　　者：周书全[1] 郑菊梅[1] 成岩[1] 

机构地区：[1]卫生部兰州生物制品研究所

出　　处：《微生物学免疫学进展》1994年第4期13-15,共3页Progress In Microbiology and Immunology

摘　　要：本文报导了乙型肝炎阳性血浆中ＨＢｓＡｇ的滴度与保存温度和疫苗收量的关系。证明：１．４─５年于─２０℃保存该血浆对ＨＢｓＡｇ滴度无明显影响；２．于─２０℃和３０℃反复冻融八次以上ＨＢｓＡｇ滴度开始下降。３．于２５℃保存５６天后，该血浆中的ＨＢｓＡｇ可下降一个滴度，提示要尽可能避免反复冻融，缩短室温保存时间。用乙肝阳性血浆制备疫苗时疫苗的收量与血浆中ＨＢｓＡｇ的滴度密切相关，当ＲＰＨＡ滴度为１：５１２时，收量明显偏低，ＲＰＨＡ滴度为１：２０４８以上时疫苗收量较高。The relationship between the HBsAg titer of positive HBsAg plasma temperatures of storage at different and yield of HB-vaccine was estimated.1.Stored at -20℃ for a long-term there was no effect on HBsAg titer.2.Freeze-thawed repeatedly over 8 times at -20℃ and 30℃ The titier began to decrease .3.Stored at 25℃ the HBsAg titer dropped down on CEP fold after 56 days.These results showed that the storing time at room temperature should be shorten and the positive HBsAg plasma should avoid freeze--thawing repeatedly. The yield of HB-vaccine relates to HBsAg titer of plasma. The higher the HBsAg titer of the positive HBsAg plasma, the higher the yield of HB-vaccine.

关 键 词：乙型肝炎 表面抗原 阳性血浆 乙型肝炎疫苗 

分 类 号：R392－33[医药卫生—免疫学] R512.620.3[医药卫生—基础医学]