果胶酶生产菌原生质体再生及诱变育种  

REGENERATION AND MUTAGENESIS OF PROTOPLASTS FRO ASPERGILLUS CARBONERIUS

作　　者：朱宝成[1] 王俊刚[1] 成亚利[1] 杨天波[1] 李庆余[1] 王谦[1] 

机构地区：[1]河北大学生物系,河北省微生物研究所

出　　处：《微生物学通报》1994年第1期15-18,共4页Microbiology China

摘　　要：用０．５％蜗牛酶和０．５％纤维素酶的混合酶液酶解２８℃下培养２０－２８小时的炭黑曲霉菌丝体，原生质体形成效为９．４８×１０￣５／ｍｌ原生质体再生率为０．２６－０．８２％。高能电子处理原生质体，筛选出了高产变异株，酶活提高了一倍。炭黑曲霉原生质体的红外吸收光谱显示了细胞内ＤＮＡ分子的吸收特征，与孢子的红外吸收特征略有差异。Agreat number 9.48×10￣5/ml)of protoplasts from mycelia Aspergillus carbonerius 3.396 incubated at 28℃ for 20-28 h was released by snail digestase 0.5%andcellulase(ONOZUK R-10) 0.5%; The regeneration frequencies of protoplasts were 0. 26-0.82%, A mutant Asp,H36 with high activity of pectate lyase was obtained by high-electronirradiation on protoplasts of the strain 3. 396.Its pectate lyase activity increased from 1500u/mlof the parent strain to 3000u/ml.IR spectrum of its protoplasts was similar to that of its DNA,but a little different from that of its spores.

关 键 词：炭黑曲霉 原生质体 果胶酶 育种 

分 类 号：Q939.97[生物学—微生物学]