紫球藻胞外多糖抗柯萨奇B_3病毒活性  

Antiviral Activity of Extracellular Polysaccharide from Porphyridium sp. Against CVB_3 Virus

作　　者：刘石生[1] 魏东[1] 朱桂芳[1] 王一飞[2] 李琳[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]暨南大学广州(暨南)生物医药研究开发基地,广东广州510632

出　　处：《华南理工大学学报（自然科学版）》2005年第2期82-85,90,共5页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家自然科学基金资助项目(20276021);广东省科技计划资助项目(2003C104025);广东省自然科学基金团队项目(200311)

摘　　要：采用离子交换凝胶QSepharoseFastFlow,以NaCl梯度洗脱,将紫球藻胞外多糖 (ESPS)分离成3个组分ESPS0、ESPS1.0和ESPS1.6,分别占粗多糖总量的44.2%(以质量 计,下同),20.3%和16.4%.ESPS0中未测出蛋白,ESPS1.0和ESPS1.6的蛋白含量分别为 7.8%和7.6%;ESPS0、ESPS1.0和ESPS1.6的硫酸基含量分别为16.3%,11.6%和8.3%.利 用体外模型测试了3个组分的抗CVB3病毒的活性.发现ESPS0,ESPS1.0和ESPS1.6抑制 CVB3病毒的半数抑制质量浓度分别为2.03,0.51和0.53mg/L,治疗指数IT值分别为 61.6,245.1和235.8;在相同条件下,阳性对照药物病毒唑的半数抑制质量浓度和IT值 分别为31.25mg/L和16.0.结果表明,3种组分抗CVB3活性均高于病毒唑.Three components, named ESPS_0, ESPS_ 1.0 and ESPS_ 1.6, were separated from extracellular polysaccharide (ESPS) of Porphyridium sp. by NaCl gradient elution with Q Sepharose Fast Flow gel. ESPS_0, ESPS_ 1.0 and ESPS_ 1.6 account for 44.2% (in mass, the same below), 20.3% and 16.4% of the total ESPS, their protein content were at 0, 7.8% and 7.6%, SO_3- radical content were 16.3%, 11.6% and 8.3%, respectively. By in vitro anti-CVB_3 activity test to the three components, the results show that their mass concentrations of half inhibition to virus were at 2.03, 0.51 and 0.53mg/L, and I_T values were at 61.6, 245.1 and 235.8, respectively. Under the same conditions, the mass concentration of half inhibition to virus and I_T value of the positive control drug Ribavirin were at 31.25mg/L and 16.0. They indicate that the anti-CVB_3 activity of all the three components were higher than Ribavirin.

关 键 词：紫球藻 多糖 柯萨奇B3病毒 抗病毒活性 

分 类 号：R282[医药卫生—中药学]