芦荟多糖对荷瘤小鼠肿瘤细胞膜功能的影响  

Study on effects of Aloe polysaccharide on tumor cell membrane function of mice bearing tumor

作　　者：邹翔[1] 孙宇[1] 王宏亮[1] 

机构地区：[1]哈尔滨商业大学药物研究所博士后科研工作站,黑龙江哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2005年第1期11-13,5,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省科技厅攻关项目(G00C201303);哈尔滨市科技局攻关项目(2003AA6CN095).

摘　　要：研究芦荟多糖对荷瘤小鼠肿瘤细胞膜功能的影响.采用荧光分光光度法、SDS-聚丙烯酰胺凝胶电泳以及唾液酸试剂盒分别测定库拉索和木立2种芦荟多糖对S180小鼠肿瘤细胞膜脂流动性、膜蛋白及唾液酸含量的影响.结果表明,2种芦荟多糖各剂量组均能不同程度地降低S180小鼠肿瘤细胞膜脂流动性、膜交联蛋白百分比含量和唾液酸的含量,且两种多糖中、高剂量组具有显著性(P<0 05或P<0 01).提示,芦荟多糖对S180小鼠肿瘤细胞膜功能具有抑制作用,提示其可能为芦荟多糖发挥抗肿瘤作用的主要机制之一.To investigate the effects of Aloe polysaccharide(AP) on tumor membrane function of S_(180) mice. Lipid fluidity is measured by Skinitzky method; the content of tumor membrane protein is analyzed By SDS-PAGE; the content of sialic acid (SA) is measured by SA reagent test Kit. The results showed that different dosage groups of the two kinds of Aloe polysaccharide can reduce the lipid fluidity, tumor SA content, decrease tumor membrane protein content at different level. While the effect of median and high dose groups of AP are remarkable (P<0.05 or P<0.01). AP can restrain S_(180) tumor membrane function, and this may be one of the most important anti-tumor mechanisms of AP.

关 键 词：肿瘤细胞膜 芦荟多糖 S180小鼠 荷瘤小鼠 唾液酸 膜功能 膜脂流动性 酸含量 剂量 蛋白 

分 类 号：R285.5[医药卫生—中药学] R730[医药卫生—中医学]