高浓度氨甲酰胆碱与去甲肾上腺素对心室肌细胞L型钙电流的联合作用  

Combination effects of high concentration carbachol and norepinephrine on L type Ca^(2+) current of cardiac ventricular myocytes

作　　者：孟华千[1] 崔香丽[2] 陈还珍[2] 赵录英[2] 吴博威[2] 

机构地区：[1]山西省卫生厅卫生监督所,太原030045 [2]山西医科大学

出　　处：《山西医科大学学报》2005年第1期5-6,共2页Journal of Shanxi Medical University

基　　金：山西省自然科学基金资助项目 ( 2 0 0 3 110 1)

摘　　要：目的　观察高浓度氨甲酰胆碱与去甲肾上腺素共存时对心室肌细胞L型钙电流的影响。方法　采用全细胞膜片钳方法记录单个大鼠心室肌细胞的L型钙电流。结果　去甲肾上腺素剂量依赖性地激动心室肌细胞L型钙电流 ,5 μmol/L去甲肾上腺素使L型钙电流由对照组的 (30 5± 132 ) pA增加到 (4 5 0± 10 8)pA(P <0 .0 5 )。氨甲酰胆碱不影响L型钙电流 ,但对去甲肾上腺素激动的L型钙电流有抑制作用。结论　高浓度的氨甲酰胆碱对L型钙电流无直接作用 。Objective To observe the combination effects of high concentration carbachol and norepinephrine on L type Ca 2+ current of cardiac ventricular myocytes. Methods L type Ca 2+ current of single cardiac ventricular myocytes in rats was recorded by whole cell patch clamp technique. Results Norepinephrine stimulated L type Ca 2+ current of cardiac ventricular myocytes dose-dependently. Norepinephrine 5 μmol/L increased L type Ca 2+ current from (305±132)pA of control to (450±108)pA(P<0.05). Carbachol had no effect on L type Ca 2+ current, while exerted inhibitory effect on L type Ca 2+ current induced by norepinephrine. Conclusion High concentration carbachol has no direct effect on L type Ca 2+ current. It inhibited L type Ca 2+ current stimulated by norepinephrine.

关 键 词：钙通道 L型 卡巴胆碱 去甲肾上腺素 心室肌细胞 大鼠 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]